Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5007777..5008339 Replicon chromosome
Accession NZ_CP069441
Organism Escherichia coli strain FDAARGOS_1261

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6J91_RS23735 Protein ID WP_000605675.1
Coordinates 5008061..5008339 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6J91_RS23730 Protein ID WP_000781370.1
Coordinates 5007777..5008061 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J91_RS23715 5002958..5006005 + 3048 WP_077634457.1 formate dehydrogenase-N subunit alpha -
I6J91_RS23720 5006018..5006902 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6J91_RS23725 5006895..5007548 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6J91_RS23730 5007777..5008061 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
I6J91_RS23735 5008061..5008339 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J91_RS23740 5008525..5009535 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6J91_RS23745 5009668..5011365 - 1698 WP_000433462.1 malate dehydrogenase -
I6J91_RS23750 5011521..5011658 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6J91_RS23755 5011760..5011975 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6J91_RS23760 5012320..5012751 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T190401 WP_000605675.1 NZ_CP069441:c5008339-5008061 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T190401 NZ_CP091327:198296-198766 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT190401 WP_000781370.1 NZ_CP069441:c5008061-5007777 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT190401 NZ_CP091327:197856-198299 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References