Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1319834..1320561 Replicon chromosome
Accession NZ_CP069441
Organism Escherichia coli strain FDAARGOS_1261

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag I6J91_RS06205 Protein ID WP_204097819.1
Coordinates 1320250..1320561 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6J91_RS06200 Protein ID WP_000126304.1
Coordinates 1319834..1320253 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J91_RS06185 1315997..1318249 - 2253 WP_001535518.1 carbamoyltransferase HypF -
I6J91_RS06190 1318402..1318929 - 528 WP_001078777.1 electron transport protein HydN -
I6J91_RS06195 1319314..1319742 + 429 WP_000536066.1 DUF4259 domain-containing protein -
I6J91_RS06200 1319834..1320253 - 420 WP_000126304.1 helix-turn-helix domain-containing protein Antitoxin
I6J91_RS06205 1320250..1320561 - 312 WP_204097819.1 type II toxin-antitoxin system HigB family toxin Toxin
I6J91_RS06210 1320724..1321479 - 756 WP_001314074.1 hypothetical protein -
I6J91_RS06215 1321522..1321989 - 468 WP_000132955.1 hydrogenase maturation peptidase HycI -
I6J91_RS06220 1321982..1322392 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
I6J91_RS06225 1322389..1323156 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
I6J91_RS06230 1323156..1323698 - 543 WP_000493800.1 formate hydrogenlyase subunit HycF -
I6J91_RS06235 1323708..1325417 - 1710 WP_001288123.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1310489..1349407 38918


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12428.14 Da        Isoelectric Point: 9.7105

>T190389 WP_204097819.1 NZ_CP069441:c1320561-1320250 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHTEYDKLTRYYRENKE

Download         Length: 312 bp

>T190389 NZ_CP091326:c236241-236023 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15474.50 Da        Isoelectric Point: 4.4596

>AT190389 WP_000126304.1 NZ_CP069441:c1320253-1319834 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT190389 NZ_CP091326:c236642-236268 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References