Detailed information of TA system TA190374

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 131500..131926
• Replicon: plasmid unnamed
• Accession: NZ_CP069439
• Organism: Escherichia coli strain FDAARGOS_1260

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J90_RS25005
• Protein ID: WP_001312861.1
• Coordinates: 131500..131658 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	131702..131926 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J90_RS24975	126897..127586	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
I6J90_RS24980	127773..128156	-	384	WP_001151538.1	relaxosome protein TraM	-
I6J90_RS24985	128477..129079	+	603	Protein_146	transglycosylase SLT domain-containing protein	-
I6J90_RS24990	129376..130197	-	822	WP_001234445.1	DUF945 domain-containing protein	-
I6J90_RS24995	130307..130603	-	297	WP_001272251.1	hypothetical protein	-
I6J90_RS25000	130995..131199	+	205	Protein_149	pilus protein	-
I6J90_RS25005	131500..131658	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	131702..131926	-	225	NuclAT_0	-	Antitoxin
-	131702..131926	-	225	NuclAT_0	-	Antitoxin
-	131702..131926	-	225	NuclAT_0	-	Antitoxin
-	131702..131926	-	225	NuclAT_0	-	Antitoxin
I6J90_RS25010	131738..131917	+	180	WP_001309233.1	hypothetical protein	-
I6J90_RS25015	131938..132657	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
I6J90_RS25020	132654..133088	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6J90_RS25025	133157..135121	-	1965	WP_204147729.1	ParB/RepB/Spo0J family partition protein	-
I6J90_RS25030	135182..135415	-	234	WP_000006003.1	DUF905 family protein	-
I6J90_RS25035	135473..136000	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A)	senB	1..168315	168315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190374 WP_001312861.1 NZ_CP069439:c131658-131500 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190374 NZ_CP091317:2479608-2479787 [Lysobacter soli]
ATGACGAAAAGCGCGGATGTGATCCGGGATCTGGAGCGGGCGGGCTGGACGCTAGCCCGAGTCAGCGGCTCGCATCACAT
CTTCACCAAACCCGGAAAGCCGCCGATTTCGGTACCGCACCCGAAGAAGGGTCTCGGTGCGGGCCTGGTCCACAAGCTGC
GCAAGCAGGCCGGCCTGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT190374 NZ_CP069439:c131926-131702 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin