Detailed information of TA system TA190370

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89158..89411
• Replicon: plasmid unnamed
• Accession: NZ_CP069439
• Organism: Escherichia coli strain FDAARGOS_1260

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: I6J90_RS24755
• Protein ID: WP_001336447.1
• Coordinates: 89158..89307 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	89355..89411 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J90_RS24715	84236..84933	-	698	Protein_92	IS1 family transposase	-
I6J90_RS24720	85182..85583	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I6J90_RS24725	85516..85773	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I6J90_RS24730	85866..86519	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I6J90_RS24735	86617..86757	-	141	WP_001333237.1	hypothetical protein	-
I6J90_RS24740	87458..88315	-	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
I6J90_RS24745	88308..88382	-	75	WP_001365705.1	RepA leader peptide Tap	-
I6J90_RS24750	88616..88873	-	258	WP_000084404.1	replication regulatory protein RepA	-
I6J90_RS24755	89158..89307	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	89355..89411	+	57	NuclAT_1	-	Antitoxin
-	89355..89411	+	57	NuclAT_1	-	Antitoxin
-	89355..89411	+	57	NuclAT_1	-	Antitoxin
-	89355..89411	+	57	NuclAT_1	-	Antitoxin
I6J90_RS24760	89506..89943	-	438	WP_000872609.1	hypothetical protein	-
I6J90_RS24765	90096..90479	-	384	WP_001109264.1	hypothetical protein	-
I6J90_RS24770	90582..91043	-	462	WP_000760080.1	thermonuclease family protein	-
I6J90_RS24775	91796..91999	-	204	WP_001336517.1	hypothetical protein	-
I6J90_RS24780	92151..92708	-	558	WP_000139329.1	fertility inhibition protein FinO	-
I6J90_RS24785	92763..93509	-	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A)	senB	1..168315	168315
	-	flank	IS/Tn	-	-	84236..84613	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T190370 WP_001336447.1 NZ_CP069439:c89307-89158 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T190370 NZ_CP091311:c1747129-1746836 [Pseudomonas juntendi]
ATGACACAAACCTTCTCTGAATTCGATCCCGCCGAATACCTCACCACTCCTGAAGCAATCGCCGTGTTCATGACTGACGC
CCTGGAGACCGGGGATGCCCGCTATGTCGCCAAAGCTGTCGGCGTAGTAGCGCGTGCCAAAGGCATGAGCGAGCTGGCAA
AAGACACAGGGCTATCTCGTGAACAGCTTTACCGGTCGTTCAGCGAGCATGGCAACCCAACCCTCAAATCGTTCCTGACC
GTGATGAAAGCATTGGGTATACACATGACAGCGCGTCCTCACATCGCAGGCTGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT190370 NZ_CP069439:89355-89411 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin