Detailed information of TA system TA190354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1650953..1651680
• Replicon: chromosome
• Accession: NZ_CP069438
• Organism: Escherichia coli strain FDAARGOS_1260

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: I6J90_RS08190
• Protein ID: WP_000547563.1
• Coordinates: 1651369..1651680 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J90_RS08185
• Protein ID: WP_000126304.1
• Coordinates: 1650953..1651372 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J90_RS08170	1647116..1649368	-	2253	WP_001535518.1	carbamoyltransferase HypF	-
I6J90_RS08175	1649521..1650048	-	528	WP_001078777.1	electron transport protein HydN	-
I6J90_RS08180	1650433..1650861	+	429	WP_000536066.1	DUF4259 domain-containing protein	-
I6J90_RS08185	1650953..1651372	-	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
I6J90_RS08190	1651369..1651680	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6J90_RS08195	1651843..1652598	-	756	WP_001314074.1	hypothetical protein	-
I6J90_RS08200	1652641..1653108	-	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
I6J90_RS08205	1653101..1653511	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6J90_RS08210	1653508..1654275	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6J90_RS08215	1654275..1654817	-	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
I6J90_RS08220	1654827..1656536	-	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T190354 WP_000547563.1 NZ_CP069438:c1651680-1651369 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T190354 NZ_CP091302:122195-122467 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT190354 WP_000126304.1 NZ_CP069438:c1651372-1650953 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT190354 NZ_CP091302:121932-122195 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCATGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22