Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3675182..3675876 Replicon chromosome
Accession NZ_CP069386
Organism Escherichia coli strain XH1815

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JSW75_RS17725 Protein ID WP_001263489.1
Coordinates 3675478..3675876 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JSW75_RS17720 Protein ID WP_000554758.1
Coordinates 3675182..3675475 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSW75_RS17700 (3670814) 3670814..3671311 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JSW75_RS17705 (3671535) 3671535..3673247 - 1713 Protein_3451 flagellar biosynthesis protein FlhA -
JSW75_RS17710 (3673219) 3673219..3674004 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JSW75_RS17715 (3674075) 3674075..3675130 + 1056 WP_001226164.1 DNA polymerase IV -
JSW75_RS17720 (3675182) 3675182..3675475 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JSW75_RS17725 (3675478) 3675478..3675876 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JSW75_RS17730 (3675886) 3675886..3676338 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
JSW75_RS17735 (3676656) 3676656..3676862 + 207 Protein_3457 RtcB family protein -
JSW75_RS17740 (3676858) 3676858..3677379 + 522 Protein_3458 peptide chain release factor H -
JSW75_RS17745 (3677436) 3677436..3678893 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JSW75_RS17750 (3679154) 3679154..3679612 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (3680208) 3680208..3680288 + 81 NuclAT_12 - -
- (3680208) 3680208..3680288 + 81 NuclAT_12 - -
- (3680208) 3680208..3680288 + 81 NuclAT_12 - -
- (3680208) 3680208..3680288 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T190324 WP_001263489.1 NZ_CP069386:3675478-3675876 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T190324 NZ_CP091244:2344740-2345018 [Thiothrix winogradskyi]
ATGATCATTAGCTTTATTCATAAAGGTTTAGAACGCTTTTACCTGACAGGTAGTAAAGCAGGTATTCAACCTGAACACGC
TCCGAAATTGCGTCGAATTCTGACCGTTTTGGAAACAGCCCAGATACCAGATGATATGGATTTGCCCGGCTACCGACTTC
ATCCCCTGCAAGGCAATAGACAAGGAACATGGTCTGTTAGGGTCAGCGGTAACTGGCTTATTACGTTTCAATTTACTGAG
CAGAATGTTGAACTTGTCGATTATGAAGACTATCACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT190324 WP_000554758.1 NZ_CP069386:3675182-3675475 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT190324 NZ_CP091244:2345030-2345293 [Thiothrix winogradskyi]
ATGCCGATGTATGATCCTGCCCATCCGGGTGAAATCCTTGGAGAAGATATTCTCAAAGAGTTAAATATAACCATTACCGC
AGCCGCTGAAAAACTTGGCATTAGCCGGAAAACACTTTCTGCTATCGTTAATGGTCATGCCCCCATTACAGCAGAAACCG
CTATCAAATTGGAGAAAGTATTTGGCAAGCCTGATGCTGAGCATTGGCTACGTATGCAAATGACTTATGATTTGTATCAT
GCTCGTCAGAAAATGGCGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References