Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3669138..3669679 Replicon chromosome
Accession NZ_CP069386
Organism Escherichia coli strain XH1815

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JSW75_RS17685 Protein ID WP_000615983.1
Coordinates 3669138..3669416 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JSW75_RS17690 Protein ID WP_000729703.1
Coordinates 3669419..3669679 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSW75_RS17670 (3666720) 3666720..3667298 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
JSW75_RS17675 (3667504) 3667504..3668271 + 768 WP_000333380.1 class II glutamine amidotransferase -
JSW75_RS17680 (3668242) 3668242..3668982 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JSW75_RS17685 (3669138) 3669138..3669416 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JSW75_RS17690 (3669419) 3669419..3669679 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JSW75_RS17695 (3669889) 3669889..3670638 + 750 WP_000056849.1 C40 family peptidase -
JSW75_RS17700 (3670814) 3670814..3671311 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JSW75_RS17705 (3671535) 3671535..3673247 - 1713 Protein_3451 flagellar biosynthesis protein FlhA -
JSW75_RS17710 (3673219) 3673219..3674004 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T190323 WP_000615983.1 NZ_CP069386:c3669416-3669138 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T190323 NZ_CP091244:2032218-2032796 [Thiothrix winogradskyi]
ATGTTTTCAGGCACAAACTTTACCGCCTTGTACGACGCTTGTGTCCTGTATCCTGCCCCGCTACGTGATTTGCTGGTTAG
ACTGGCGCATACAGGGACGTTCCGGGCGCGTTGGACACAACAAATTCATGAGGAATGGATACGCAATGCTTTGCGCAACA
ATCCCCGCCTGACCCGTGAACGGCTTGAAGCCACTGCCAGCAAAATGAATGACGCCATCATGGATTCATTGGTGTCAGAC
TACGCGCCGCTCATTGCAGGGTTGGAACTGCCGGATACGAATGACAGGCATGTACTGGCGGCGGCCATCAAAGGGCAGGC
GGAGGTCATTGTCACCTTTAACCTGAAAGACTTCCCGGATCGCTGCCTGCATCCATTCAATATCTGGGCGCAGCACCCTG
ATGAGTTCATCAGCCACCTAATAGACCTGTCACCTGCCACGGTATTGCTGGCGGCAAGGCAACAACGGGCTGCATTGAAA
AATCCACCACGCACCGTCGATGAGTTTCTGGATACGCTGCTCCGCCAACAGTTGCCCCGTACAGTCGCTTTCCTGAAAGA
GCGTAAGGATTTGATTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT190323 WP_000729703.1 NZ_CP069386:c3669679-3669419 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT190323 NZ_CP091244:2031739-2032218 [Thiothrix winogradskyi]
ATGCGAAGAGAACAACCCAAGGAGTTTGCAGCCATGTCTGCCTTAATCAGCTACCAAATCCCAACCTCGGAGGATGCCCG
GCTGGCGGTGGAAAGCAGCCGTTTGCTGGCTACTTGTATCGGCAAGGGGGAAAGTGCCTGCTTGCGGCTGCGCGAGGGGG
ATGAAATATTACAAGTCCCGGTCAAAGCCATCCGTCTACTGGTGGACATTCTGGAAGCCATGGCGCGAGGGGATGCGGTG
TCCCTTATCCCGATCCACAAGGAACTGACGACCCAGGAAGCCGCCAATCTCCTCAACGTGTCACGCCCCTATCTGGTGAA
ACTGCTGGAAAAGGGGGACATTCCTTTCCACAAGAACGGCGTGCGCCGCAAGGTGCTGTTCAAGGATCTGATGGAATACA
AACAGCAACGTGACCAGAGCACTAGGGAGCTGTTGGATGACCTGACTGCCGAGGCGCAGGAACTGGATATGGGGTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References