Detailed information of TA system TA190287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4594704..4595458
• Replicon: chromosome
• Accession: NZ_CP069381
• Organism: Salmonella enterica strain GSJ/2016-Sal-016

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: JSR10_RS22320
• Protein ID: WP_000558166.1
• Coordinates: 4594704..4595015 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JSR10_RS22325
• Protein ID: WP_001259011.1
• Coordinates: 4595012..4595458 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSR10_RS22290 (4590362)	4590362..4591264	+	903	WP_000331362.1	formate dehydrogenase subunit beta	-
JSR10_RS22295 (4591261)	4591261..4591896	+	636	WP_000829028.1	formate dehydrogenase cytochrome b556 subunit	-
JSR10_RS22300 (4591893)	4591893..4592822	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
JSR10_RS22305 (4592869)	4592869..4593159	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
JSR10_RS22310 (4593160)	4593160..4593471	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
JSR10_RS22315 (4593689)	4593689..4594618	+	930	WP_001127703.1	alpha/beta hydrolase	-
JSR10_RS22320 (4594704)	4594704..4595015	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
JSR10_RS22325 (4595012)	4595012..4595458	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
JSR10_RS22330 (4595473)	4595473..4596414	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
JSR10_RS22335 (4596459)	4596459..4596896	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
JSR10_RS22340 (4596893)	4596893..4597765	-	873	WP_000921427.1	virulence factor BrkB family protein	-
JSR10_RS22345 (4597759)	4597759..4598358	-	600	WP_000965695.1	glucose-1-phosphatase	-
JSR10_RS22350 (4598549)	4598549..4599352	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
JSR10_RS22355 (4599386)	4599386..4600282	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T190287 WP_000558166.1 NZ_CP069381:4594704-4595015 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T190287 NZ_CP091231:c2854204-2853863 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT190287 WP_001259011.1 NZ_CP069381:4595012-4595458 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT190287 NZ_CP091231:c2854434-2854204 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90