Detailed information of TA system TA190253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1047132..1047725
• Replicon: chromosome
• Accession: NZ_CP069353
• Organism: Saccharopolyspora erythraea strain NRRL 23338

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2A9IZT4
• Locus tag: JQX30_RS04780
• Protein ID: WP_029621452.1
• Coordinates: 1047132..1047413 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQX30_RS04785
• Protein ID: WP_011873183.1
• Coordinates: 1047426..1047725 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQX30_RS04760	1042492..1043523	+	1032	WP_009944211.1	ABC transporter ATP-binding protein	-
JQX30_RS04765	1043516..1044517	+	1002	WP_009944207.1	dipeptide ABC transporter ATP-binding protein	-
JQX30_RS04770	1044769..1046247	-	1479	WP_009944204.1	peptidase S10	-
JQX30_RS04775	1046332..1047078	+	747	WP_037303372.1	uracil-DNA glycosylase	-
JQX30_RS04780	1047132..1047413	+	282	WP_029621452.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQX30_RS04785	1047426..1047725	+	300	WP_011873183.1	HigA family addiction module antidote protein	Antitoxin
JQX30_RS04790	1048089..1048895	-	807	WP_009944200.1	metalloregulator ArsR/SmtB family transcription factor	-
JQX30_RS04795	1049402..1050724	+	1323	WP_009944198.1	NAD(P)/FAD-dependent oxidoreductase	-
JQX30_RS04800	1051052..1052206	+	1155	WP_009944195.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11019.53 Da
• Isoelectric Point: 10.2071

>T190253 WP_029621452.1 NZ_CP069353:1047132-1047413 [Saccharopolyspora erythraea]
MLRSFADKETERVWQRRRSRMLDPKVQRAALRKLAILDAAETVEDLRVPPGNQLEKLNGDRVGQYSIRINQQWRIRFEWT
SAGPENVEIVDYH

Nucleotide

• Length: 282 bp

>T190253 NZ_CP091199:26960-27235 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11207.81 Da
• Isoelectric Point: 5.6335

>AT190253 WP_011873183.1 NZ_CP069353:1047426-1047725 [Saccharopolyspora erythraea]
MSDECVMPPVHPGEVLAEEYLEPLGVTQHRLAVAIGVPPRRINEIVHGKRGISADTALRLARFFGTSERFWLNLQSRYDL
ERERDALADTLDRIQPLSV

Nucleotide

• Length: 300 bp

>AT190253 NZ_CP091199:26701-26967 [Enterococcus faecalis]
TTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACTATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAACGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A2A9IZT4