Detailed information of TA system TA190238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5293305..5293900
• Replicon: chromosome
• Accession: NZ_CP069336
• Organism: Pseudomonas aeruginosa strain E01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ453_RS24550
• Protein ID: WP_003113526.1
• Coordinates: 5293622..5293900 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ453_RS24545
• Protein ID: WP_003113527.1
• Coordinates: 5293305..5293610 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ453_RS24525 (JQ453_24510)	5289381..5289638	+	258	WP_010793759.1	ATP-binding protein	-
JQ453_RS24530 (JQ453_24515)	5290014..5290877	-	864	WP_010793758.1	integrase domain-containing protein	-
JQ453_RS24535 (JQ453_24520)	5291479..5292621	-	1143	WP_010793757.1	STY4528 family pathogenicity island replication protein	-
JQ453_RS24545 (JQ453_24530)	5293305..5293610	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ453_RS24550 (JQ453_24535)	5293622..5293900	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ453_RS24555	5293953..5294081	-	129	Protein_4860	integrase	-
JQ453_RS24560 (JQ453_24540)	5294229..5296457	+	2229	WP_003099265.1	TonB-dependent receptor	-
JQ453_RS24565 (JQ453_24545)	5296527..5297174	-	648	WP_003095021.1	carbonate dehydratase	-
JQ453_RS24570 (JQ453_24550)	5297236..5298474	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190238 WP_003113526.1 NZ_CP069336:c5293900-5293622 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190238 NZ_CP091188:26847-27137 [Vibrio anguillarum]
ATGAATATTACCAGGCTCGATGATTTTGATCCTGCTGACTATCTTGATAATGCTGAATCTATACAGGCATATATTGATGA
GGCGGTAGCAACTGGTGATGCTGCTTTTATTAGTCAATCCTTGGGTGTTATAGCCCGTGCCAAAGGTATGAATACGCTAG
CAGAGGAAACCGGTTTATCTCGTGAAACTTTATATAGAACATTAAGCGTTAAAGGGAATCCTAACCTTAAAACGCTTATC
TCTATCACTAATGCGCTTGGTGTATCTGTCAAACTCGGCTCTTACTGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190238 WP_003113527.1 NZ_CP069336:c5293610-5293305 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190238 NZ_CP091188:26527-26823 [Vibrio anguillarum]
ATGTTCACTATAAAGCGCACGCAACTTTTTGATGATTGGTTGAAGACACTTAAAGATGCTCAGGCACGAGGTGCTATCAC
TGCTCGTGTTCAGCGCCTTACTCAGGGTTTGAGTGGGGATGTTAAGCCGGTTGGTTCGGGCATTAGTGAGCTTAGGATAC
ATGTCGGTAAAGGTTATCGAGTTTATTTTAAGCGTAGTGGAAAACAGATCGTTGTCTTACTTTGCGGTGGCAATAAGAAA
ACTCAGAATCAAGATATCGAACGCGCTAAAGAGTTGGTTCAATATTTTGATTTGTAG

Structures

Toxin

• AlphaFold DB: V6ALY3