Detailed information of TA system TA190232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5307540..5308135
• Replicon: chromosome
• Accession: NZ_CP069335
• Organism: Pseudomonas aeruginosa strain E02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ454_RS24650
• Protein ID: WP_003113526.1
• Coordinates: 5307857..5308135 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ454_RS24645
• Protein ID: WP_003113527.1
• Coordinates: 5307540..5307845 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ454_RS24610 (JQ454_24600)	5302570..5302842	-	273	WP_124117649.1	DNA-binding protein	-
JQ454_RS24615 (JQ454_24605)	5302952..5303218	+	267	WP_023088595.1	hypothetical protein	-
JQ454_RS24620 (JQ454_24610)	5303274..5303609	+	336	WP_124117651.1	hypothetical protein	-
JQ454_RS24625 (JQ454_24615)	5303641..5304609	-	969	WP_124117653.1	hypothetical protein	-
JQ454_RS24630 (JQ454_24620)	5304606..5305703	-	1098	WP_235366366.1	ATP-binding protein	-
JQ454_RS24635 (JQ454_24625)	5305733..5305972	-	240	WP_124126190.1	hypothetical protein	-
JQ454_RS24640 (JQ454_24630)	5306105..5307211	+	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ454_RS24645 (JQ454_24635)	5307540..5307845	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ454_RS24650 (JQ454_24640)	5307857..5308135	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ454_RS24655	5308188..5308316	-	129	Protein_4880	integrase	-
JQ454_RS24660 (JQ454_24645)	5308464..5310692	+	2229	WP_003099265.1	TonB-dependent receptor	-
JQ454_RS24665 (JQ454_24650)	5310762..5311409	-	648	WP_003095021.1	carbonate dehydratase	-
JQ454_RS24670 (JQ454_24655)	5311471..5312709	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190232 WP_003113526.1 NZ_CP069335:c5308135-5307857 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190232 NZ_CP091186:c134629-134351 [Vibrio anguillarum]
ATGATTTTATGGGAAGAGGAATCGCTAAATGATCGTGAGAAGATCTTTGAGTTTCTTTATGACTTTAACCCTGATGCGGC
TGAAAAAACTGATGACATTATTGAGGCTAAAGTAGAAAACTTACTCGAACAACCATTGATGGGTGTTCAACGGGATGGAA
TTCGAGGACGATTACTCATTATTCCCGAAATCTCAATGATCGTTTCGTACTGGGTAGAAGGTTCAATTATTCGCATAATG
CGAGTTCTACACCAGAAGCAGAAATTCCCTACCGATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190232 WP_003113527.1 NZ_CP069335:c5307845-5307540 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190232 NZ_CP091186:c134910-134626 [Vibrio anguillarum]
ATGGACACTCGAATTCAATTTCGTGTTGATGACGAAATTAAACGTTTAGCCCAACAAATGGCTGAGAGCCAAGGTCGCAC
ACTCAGCGATGCTTGCCGTGAACTTACTGAACAAATGGCTGAGCAACAAAGAAAAACATTATCTCATGACGCTTGGTTAA
CTGAACAAATAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTTCATTTGTTGAACATAATTCAGCTAAAGCACGA
ATGGCTGAACGAAAGGCCAAAATTCGTAATCGAGGCCAGCAATGA

Structures

Toxin

• AlphaFold DB: V6ALY3