190226

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5293305..5293900 Replicon chromosome
Accession NZ_CP069334
Organism Pseudomonas aeruginosa strain E03

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag JQ455_RS24545 Protein ID WP_003113526.1
Coordinates 5293622..5293900 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JQ455_RS24540 Protein ID WP_003113527.1
Coordinates 5293305..5293610 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQ455_RS24520 (JQ455_24515) 5289381..5289638 + 258 WP_010793759.1 ATP-binding protein -
JQ455_RS24525 (JQ455_24520) 5290014..5290877 - 864 WP_010793758.1 integrase domain-containing protein -
JQ455_RS24530 (JQ455_24525) 5291479..5292621 - 1143 WP_010793757.1 STY4528 family pathogenicity island replication protein -
JQ455_RS24540 (JQ455_24535) 5293305..5293610 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
JQ455_RS24545 (JQ455_24540) 5293622..5293900 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JQ455_RS24550 5293953..5294081 - 129 Protein_4859 integrase -
JQ455_RS24555 (JQ455_24545) 5294229..5296457 + 2229 WP_003099265.1 TonB-dependent receptor -
JQ455_RS24560 (JQ455_24550) 5296527..5297174 - 648 WP_003095021.1 carbonate dehydratase -
JQ455_RS24565 (JQ455_24555) 5297236..5298474 - 1239 WP_010793756.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T190226 WP_003113526.1 NZ_CP069334:c5293900-5293622 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T190226 NZ_CP091176:c2046036-2044726 [Aeromonas caviae]
ATGGCCAGCCTGACGGTCTACATGAACGGATACCGGGTAGGTACCTTTACCAAGGCGACCAATGGTGCTCACCAATTTCA
GTACGATGAATCGTGGGTGAAGTTGCCAGGCAGCCGCCCCATCTCGCTCTCCATGCCATTACGCCAGCAGCGCTACCAGG
GCGATGAGGCCTATAACTTCTTCGATAATCTGCTGCCAGATAACCCGGAAGTAAGAAACCGGGTGGTGGCGCGCTATCAA
GCCGATTCCACCCAACCCTTTGATCTGCTCAGTTGTATTGGCCAGGACAGCGTGGGGGCATTGCAACTGGTGGCTCAGGG
CAGACCTGTGCCAGACGTTAAGCGCATTGAGTGCAAGCCGCTCTCTGACGCCGAACTGGAGCAGATCATGACCAGCTACC
AGCAAGGGATCCCGCTTGGTATGGTGAGGGAAGAGGACGATTTTCGGATATCCATCGCCGGGGCTCAGGAAAAGACCGCG
TTACTCTACCTCGACAACCGTTGGTGCTTACCGCATGCAGCGACCCCAACCACCCACATTATCAAGCTGCCCATTGGCAA
GATCGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACCCTGATCGCCAAAGCAT
TGGGTCTTCCGGTTCCCCATTGCTTCATCATGCAGGTCGGCAAGGTCAAGGCTCTGGCGGTGGAACGCTTCGATCGGCGC
TACGCCACGGATCGCAGTTGGATCATGCGCCTGCCCCAAGAGGACTTCTGCCAGGTGTTGAATGTACCGTCAGCACGCAA
GTACGAGAGCCATGGTGGACCGGGCATTGCTGACATCATGACGACCCTGCTGGGATCGGCCACGCCGGAGCAGGATCGGT
ACCTGTTTATGAAGTCTCAGGTGTTGTTCTGGCTGCTGGCTGCCACTGATGGCCATGCCAAGAATTTCTCGGTGTTTATC
GAACCTGAAGGGCGTTTCCGGCTTACACCGTTTTACGACATCCTTTCCATGTATCCTGCATTTGGTGGTAAAGGATTACA
TCCCCGTGATGCCAAGTTGGCCATGGGGCTTACTGCAACCAAGGGCAAAAAATACGCGATAGAGCAGATATTTCCTCGCC
ATTTTTATCAAACGGCCAAGGCGATTGGTTTTGAACAAGTGCAGATGGAAAAAATCATCAGCGAACTGACGGATAATCTG
GATGAGGTAATATCAATCGTTCGTCAGCAATTGCCTGATACATTTCCCACTGAGATTGCAGATTCAATTTTGGGTGGACT
CAGTTCGAAAGCGCAACGCTTAAAACGATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT190226 WP_003113527.1 NZ_CP069334:c5293610-5293305 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT190226 NZ_CP091176:c2046290-2046036 [Aeromonas caviae]
ATGAAAGTAACCAGCGCCAAGCAACTTAGTGCCTGTCTCAAGGATGCCAGGCTTTCGCAAAAGCTCTCCCAAGGCAAAGT
GGCCGGCAAGGTGGGGATCAGGCAGGACACGGTGTCCAGCTTCGAGCTCAATCCCGAGTCAACCAAGCTGGAGACCTTGT
TCAAGATCCTCGCCGCACTGGAACTCTCCCTCGATATCAGGCCCCGCACCGATCCTGCGGAAAACGCCACGCCTGGCTGG
AAGGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References