Detailed information of TA system TA190220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5247664..5248259
• Replicon: chromosome
• Accession: NZ_CP069333
• Organism: Pseudomonas aeruginosa strain R01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ457_RS24460
• Protein ID: WP_003113526.1
• Coordinates: 5247981..5248259 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ457_RS24455
• Protein ID: WP_003113527.1
• Coordinates: 5247664..5247969 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ457_RS24425 (JQ457_24385)	5242693..5242965	-	273	WP_124117649.1	DNA-binding protein	-
JQ457_RS24430 (JQ457_24390)	5243075..5243341	+	267	WP_023088595.1	hypothetical protein	-
JQ457_RS24435 (JQ457_24395)	5243397..5243732	+	336	WP_124117651.1	hypothetical protein	-
JQ457_RS24440 (JQ457_24400)	5243764..5244732	-	969	WP_124117653.1	hypothetical protein	-
JQ457_RS24445 (JQ457_24405)	5244729..5246096	-	1368	WP_124117655.1	ATP-binding protein	-
JQ457_RS24450 (JQ457_24410)	5246229..5247335	+	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ457_RS24455 (JQ457_24415)	5247664..5247969	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ457_RS24460 (JQ457_24420)	5247981..5248259	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ457_RS24465	5248312..5248440	-	129	Protein_4841	integrase	-
JQ457_RS24470 (JQ457_24425)	5248588..5250816	+	2229	WP_124120912.1	TonB-dependent receptor	-
JQ457_RS24475 (JQ457_24430)	5250886..5251533	-	648	WP_003095021.1	carbonate dehydratase	-
JQ457_RS24480 (JQ457_24435)	5251595..5252833	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190220 WP_003113526.1 NZ_CP069333:c5248259-5247981 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190220 NZ_CP091172:1334144-1334800 [Acinetobacter baumannii]
ATGGTTGGACGTAATTATAGTGATGTACTCAATCCGCAAAAGGCATTGATTTGGCGTATTGTGCATCGTGATAATATTCC
GTGGATATTAGAAAATGGCTTACATAGCAGCAATAGTCCAACACAATGTGTAAATTGGGTTCAGATTGGCAGTCCAGAAC
TGATTGAGAAAAGACAACTGCATCCTGTTCCTGTAGGGAAAAAGGGTGTGCTAAGTGACTATATTCCATTTTATTTCACA
CCATTTTCACCTATGCTGCTGAATATAAAGAGTGGCCGTGGCGGTGTACGTCAGCGTACTAATGATGAAATTGTTATTTT
AGTTTCTAGTTTGTATCAGCTACAGCAGCAGCAAATTCCTTTTGTTTTTACAAATAGTCATGCCTATTACCAATGGGCTG
AATTCTATACAGATTTATCTAGCTTAAATGAGATCGATTGGTCAATATTACAGGCAAGAGATTTTAAAAGAGATATGGAT
GATCCTGCGAAGTTTGAACGTTATCAAGCCGAAGCGTTAGTTCATCAACACTGCCCAACAAAAGTATTGATTGGGGTGAT
ATGTTATACTGAAACAGTTAAAGCTCAAATAGAACAGTGGCTGCAACAAGCAGATGTTAATATTCCTGTGCATGTGAGAC
AAGGGGTGTATTTCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190220 WP_003113527.1 NZ_CP069333:c5247969-5247664 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190220 NZ_CP091172:1334800-1335885 [Acinetobacter baumannii]
ATGATTCGTTATACAACAGGAAACTTATTAGATGCTCCTGTAGAAGCATTGGTAAATACCGTGAATACCGTTGGTGTAAT
GGGTAAAGGTATTGCTTTAATGTTTAAGGAGCGTTTTCCAAAAAACATGGCAGCTTATAGTCAAGCGTGCAAAGCTGGAC
AGGTTACAACTGGAAAAATGTTTGTGACTGAAACTAATGAGTTAATGGGCGCACGTTGGATTATTAACTTTCCAACTAAA
CAGCATTGGCGGGATAAATCCAAACTCCAATGGATTGAAGATGGTTTGCAGGATCTTAAAAAGTTCATCATTGATCATCA
AGTTAAATCTATTGCAATTCCACCATTAGGTGCGGGTAATGGTGGGTTAGAGTGGAGTCGTGTTAAATCAAAAATTGAGA
CAGAATTATCCGATTTAGAGTATGTGGATATTTTGATTTTTGAACCAACCACACAGTATCAAAACGTGGCAAAGAAAATA
GGTGTATCACAACTTACACCAGCACGAGCCATGATTGCAGAGTTAGTACGTCGTTATTGGGTATTGGGGATTGAATGTAG
TCTATTAGAAATCCAAAAACTGGCATGGTTTTTACAGCGAGTTATTGAGCAAAAACAATTACCAAATGAATTGAAGCTTA
ATTTTCAGGCGAACTATTATGGTCCTTATGCTTCTAATTTAGACCATTTGTTGAATGCAATGGATGGTAGCTATCTCAAA
TCGGACAAGCGAATTCCTGATTGTGACCCCTTAGATGTGATTTGGTTCAATGATCAGCAAAAAAGTAAAGTTCAAGGTTA
TCTGATTAGTGAAGCAAAAGAGTATATGGCTGCTGTAGAAGGAGCTGCAACATTGATAGAGGGTTTTGAATCACCATTTG
GTATGGAGCTTTTGGCAACAGTAGATTGGCTTATCTATAAGGAAGGTTATCAACCTAATGTTGAATCAATTCAAGAAGGG
TTAGCGAATTGGAGTGCAGGTGAGCGTTGGGCAAGACGGAAATCTGATTTATTCAAAGCAGAGCATATTCAATTTGCATT
ACAAAAATTAGATGGTTTTGCTTATTTTCATACAGATGTAACTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3