Detailed information of TA system TA190205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1501327..1501922
• Replicon: chromosome
• Accession: NZ_CP069331
• Organism: Pseudomonas aeruginosa strain R09

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ465_RS06945
• Protein ID: WP_003113526.1
• Coordinates: 1501327..1501605 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ465_RS06950
• Protein ID: WP_003113527.1
• Coordinates: 1501617..1501922 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ465_RS06925 (JQ465_06920)	1496753..1497991	+	1239	WP_010793756.1	C69 family dipeptidase	-
JQ465_RS06930 (JQ465_06925)	1498053..1498700	+	648	WP_003095021.1	carbonate dehydratase	-
JQ465_RS06935 (JQ465_06930)	1498770..1500998	-	2229	WP_003099265.1	TonB-dependent receptor	-
JQ465_RS06940	1501146..1501274	+	129	Protein_1368	integrase	-
JQ465_RS06945 (JQ465_06935)	1501327..1501605	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ465_RS06950 (JQ465_06940)	1501617..1501922	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ465_RS06955 (JQ465_06945)	1502251..1503357	-	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ465_RS06960 (JQ465_06950)	1503490..1504857	+	1368	WP_124117655.1	ATP-binding protein	-
JQ465_RS06965 (JQ465_06955)	1504854..1505822	+	969	WP_124117653.1	hypothetical protein	-
JQ465_RS06970 (JQ465_06960)	1505854..1506189	-	336	WP_124117651.1	hypothetical protein	-
JQ465_RS06975 (JQ465_06965)	1506245..1506511	-	267	WP_023088595.1	hypothetical protein	-
JQ465_RS06980 (JQ465_06970)	1506621..1506893	+	273	WP_124117649.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190205 WP_003113526.1 NZ_CP069331:1501327-1501605 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190205 NZ_CP091146:c66910-66533 [Pseudomonas avellanae]
GTGAAAGGCGTCCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190205 WP_003113527.1 NZ_CP069331:1501617-1501922 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190205 NZ_CP091146:c67107-66907 [Pseudomonas avellanae]
ATGAGAACGACTGTTACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCGGCTAAACGATTGATGGCTTTAGGGGCCACACTGCCAG
CGATGGAAGACATCGACCGCCGACACGAGAAGGCACTGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3