Detailed information of TA system TA190203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5247865..5248460
• Replicon: chromosome
• Accession: NZ_CP069330
• Organism: Pseudomonas aeruginosa strain R07

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ463_RS24445
• Protein ID: WP_003113526.1
• Coordinates: 5248182..5248460 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ463_RS24440
• Protein ID: WP_003113527.1
• Coordinates: 5247865..5248170 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ463_RS24410 (JQ463_24380)	5242894..5243166	-	273	WP_124117649.1	DNA-binding protein	-
JQ463_RS24415 (JQ463_24385)	5243276..5243542	+	267	WP_023088595.1	hypothetical protein	-
JQ463_RS24420 (JQ463_24390)	5243598..5243933	+	336	WP_124117651.1	hypothetical protein	-
JQ463_RS24425 (JQ463_24395)	5243965..5244933	-	969	WP_124117653.1	hypothetical protein	-
JQ463_RS24430 (JQ463_24400)	5244930..5246297	-	1368	WP_124117655.1	ATP-binding protein	-
JQ463_RS24435 (JQ463_24405)	5246430..5247536	+	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ463_RS24440 (JQ463_24410)	5247865..5248170	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ463_RS24445 (JQ463_24415)	5248182..5248460	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ463_RS24450	5248513..5248641	-	129	Protein_4839	integrase	-
JQ463_RS24455 (JQ463_24420)	5248789..5251017	+	2229	WP_003099265.1	TonB-dependent receptor	-
JQ463_RS24460 (JQ463_24425)	5251087..5251734	-	648	WP_003095021.1	carbonate dehydratase	-
JQ463_RS24465 (JQ463_24430)	5251796..5253034	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190203 WP_003113526.1 NZ_CP069330:c5248460-5248182 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190203 NZ_CP091145:c81240-80863 [Pseudomonas avellanae]
GTGAAAGGCGTCCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190203 WP_003113527.1 NZ_CP069330:c5248170-5247865 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190203 NZ_CP091145:c81437-81237 [Pseudomonas avellanae]
ATGAGAACGACTGTTACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCGGCTAAACGATTGATGGCTTTAGGGGCCACACTGCCAG
CGATGGAAGACATCGACCGCCGACACGAGAAGGCACTGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3