Detailed information of TA system TA190193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1501244..1501839
• Replicon: chromosome
• Accession: NZ_CP069329
• Organism: Pseudomonas aeruginosa strain R10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ466_RS06945
• Protein ID: WP_003113526.1
• Coordinates: 1501244..1501522 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ466_RS06950
• Protein ID: WP_003113527.1
• Coordinates: 1501534..1501839 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ466_RS06925 (JQ466_06920)	1496670..1497908	+	1239	WP_010793756.1	C69 family dipeptidase	-
JQ466_RS06930 (JQ466_06925)	1497970..1498617	+	648	WP_003095021.1	carbonate dehydratase	-
JQ466_RS06935 (JQ466_06930)	1498687..1500915	-	2229	WP_003099265.1	TonB-dependent receptor	-
JQ466_RS06940	1501063..1501191	+	129	Protein_1368	integrase	-
JQ466_RS06945 (JQ466_06935)	1501244..1501522	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ466_RS06950 (JQ466_06940)	1501534..1501839	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ466_RS06955 (JQ466_06945)	1502168..1503274	-	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ466_RS06960 (JQ466_06950)	1503407..1504774	+	1368	WP_124117655.1	ATP-binding protein	-
JQ466_RS06965 (JQ466_06955)	1504771..1505739	+	969	WP_124117653.1	hypothetical protein	-
JQ466_RS06970 (JQ466_06960)	1505771..1506106	-	336	WP_124117651.1	hypothetical protein	-
JQ466_RS06975 (JQ466_06965)	1506162..1506428	-	267	WP_023088595.1	hypothetical protein	-
JQ466_RS06980 (JQ466_06970)	1506538..1506810	+	273	WP_124117649.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190193 WP_003113526.1 NZ_CP069329:1501244-1501522 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190193 NZ_CP091143:3012348-3012623 [Pseudomonas avellanae]
ATGGAGCTGATGTGGACAAGCAAAGCGCTATCTGACGTCGCCCGCCTGTATGATTTTTTAGCGGTAGCAAACCAACCCGC
TGCCGCCCAGACCGTACAGAAGCTTACAGCGGCGCCGATCATGCTCCTTTCCAACCCGCGGATTGGTGAGCGTCTGGAAG
AGTTTGAGCCCCGGGATGTGCGCAAAATTCAGATAGGCCGCTATGAAATGCGCTACGAAATTGTGGACTCAACAATTTAT
CTGTTGCGGCTGTGGCATACACGCGAGGATCGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190193 WP_003113527.1 NZ_CP069329:1501534-1501839 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190193 NZ_CP091143:3012082-3012348 [Pseudomonas avellanae]
ATGACAACCAAGACCCGATCTGTAACAGCTCACGTACCCGAACAATTGGCTGAAAAAGTCGACCTGATGGCAGAGCGACT
CGAACGCTCTAAAAACTGGATCGTCAGACAGGCCTTGTCTGCATGGATAGATCAGGAGGAAGAACGTAGTCGCCTCACTC
GCGAAGCACTAGCCGATGTCGATGCTGGCCGCGTGATTGACCATCAGGCTGTTCAGGCCTGGGCTGACAGTTTAAGCACC
GCTACGCCTCTACCGGTTCCGCGCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3