Detailed information of TA system TA190191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5247380..5247975
• Replicon: chromosome
• Accession: NZ_CP069328
• Organism: Pseudomonas aeruginosa strain R04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ460_RS24435
• Protein ID: WP_003113526.1
• Coordinates: 5247697..5247975 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ460_RS24430
• Protein ID: WP_003113527.1
• Coordinates: 5247380..5247685 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ460_RS24400 (JQ460_24380)	5242409..5242681	-	273	WP_124117649.1	DNA-binding protein	-
JQ460_RS24405 (JQ460_24385)	5242791..5243057	+	267	WP_023088595.1	hypothetical protein	-
JQ460_RS24410 (JQ460_24390)	5243113..5243448	+	336	WP_124117651.1	hypothetical protein	-
JQ460_RS24415 (JQ460_24395)	5243480..5244448	-	969	WP_124117653.1	hypothetical protein	-
JQ460_RS24420 (JQ460_24400)	5244445..5245812	-	1368	WP_124117655.1	ATP-binding protein	-
JQ460_RS24425 (JQ460_24405)	5245945..5247051	+	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ460_RS24430 (JQ460_24410)	5247380..5247685	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ460_RS24435 (JQ460_24415)	5247697..5247975	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ460_RS24440	5248028..5248156	-	129	Protein_4837	integrase	-
JQ460_RS24445 (JQ460_24420)	5248304..5250532	+	2229	WP_003099265.1	TonB-dependent receptor	-
JQ460_RS24450 (JQ460_24425)	5250602..5251249	-	648	WP_003095021.1	carbonate dehydratase	-
JQ460_RS24455 (JQ460_24430)	5251311..5252549	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190191 WP_003113526.1 NZ_CP069328:c5247975-5247697 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190191 NZ_CP091143:661-1086 [Pseudomonas avellanae]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCTGGTATTGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGGTCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGA
ACGTCATTAACCCCTGGGAAGCGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190191 WP_003113527.1 NZ_CP069328:c5247685-5247380 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190191 NZ_CP091143:407-664 [Pseudomonas avellanae]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Structures

Toxin

• AlphaFold DB: V6ALY3