Detailed information of TA system TA190185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5246488..5247083
• Replicon: chromosome
• Accession: NZ_CP069327
• Organism: Pseudomonas aeruginosa strain R03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ459_RS24445
• Protein ID: WP_003113526.1
• Coordinates: 5246805..5247083 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ459_RS24440
• Protein ID: WP_003113527.1
• Coordinates: 5246488..5246793 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ459_RS24410 (JQ459_24380)	5241517..5241789	-	273	WP_124117649.1	DNA-binding protein	-
JQ459_RS24415 (JQ459_24385)	5241899..5242165	+	267	WP_023088595.1	hypothetical protein	-
JQ459_RS24420 (JQ459_24390)	5242221..5242556	+	336	WP_124117651.1	hypothetical protein	-
JQ459_RS24425 (JQ459_24395)	5242588..5243556	-	969	WP_124117653.1	hypothetical protein	-
JQ459_RS24430 (JQ459_24400)	5243553..5244920	-	1368	WP_124117655.1	ATP-binding protein	-
JQ459_RS24435 (JQ459_24405)	5245053..5246159	+	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ459_RS24440 (JQ459_24410)	5246488..5246793	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ459_RS24445 (JQ459_24415)	5246805..5247083	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ459_RS24450	5247136..5247264	-	129	Protein_4839	integrase	-
JQ459_RS24455 (JQ459_24420)	5247412..5249640	+	2229	WP_003099265.1	TonB-dependent receptor	-
JQ459_RS24460 (JQ459_24425)	5249710..5250357	-	648	WP_003095021.1	carbonate dehydratase	-
JQ459_RS24465 (JQ459_24430)	5250419..5251657	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190185 WP_003113526.1 NZ_CP069327:c5247083-5246805 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190185 NZ_CP091138:c2092122-2091829 [Microbacterium sp. KUDC0406]
GTGATCGACTACAGGCTCTCCCCGGCGGCGCAGCGGGATCTGTCCTCGATCTGGGACTACACGCTCGAGCGCTGGGACGA
GAGCCAGGCAGAGACCGATGTGCTGGAGATCAGGGCCGCCATCGAGCGCATCGCCGCGCAGCCTGAGCGTGGCCGCCCGG
TCGACGAGATCCGTGGGGGCTACTGGCGCTACTCGATCGGCAGCCACCTGATCTTCTTCGTCGAGCGCCCCGGCGGCGGC
GTGGACGTGATCCGCATCCTGCACCAGCGGATGGACCCCACTCGTCACGTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190185 WP_003113527.1 NZ_CP069327:c5246793-5246488 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190185 NZ_CP091138:c2092352-2092119 [Microbacterium sp. KUDC0406]
ATGGCGTCCAACACCTCGATCAGCCTCGACGAGCACTTCACGGACTTCCTCGCCCAAGAGGTCGCGACCGGGCGGTACCG
CTCCGCCAGCGAGGTCGTGCGCGCAGGGTTGCGGCTGCTGGAGGATCACGAATCGCAGATGTCCGTGCTCCGTGCATCTC
TCGCCGCCGGAGAAGAGAGCGGTGAGCCCGAGGCCTTCGACTTCGACGCGTTCATCGCATCGAAGCGCGCGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3