Detailed information of TA system TA190179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5247624..5248219
• Replicon: chromosome
• Accession: NZ_CP069326
• Organism: Pseudomonas aeruginosa strain R11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ467_RS24430
• Protein ID: WP_003113526.1
• Coordinates: 5247941..5248219 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ467_RS24425
• Protein ID: WP_003113527.1
• Coordinates: 5247624..5247929 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ467_RS24395 (JQ467_24370)	5242653..5242925	-	273	WP_124117649.1	DNA-binding protein	-
JQ467_RS24400 (JQ467_24375)	5243035..5243301	+	267	WP_023088595.1	hypothetical protein	-
JQ467_RS24405 (JQ467_24380)	5243357..5243692	+	336	WP_124117651.1	hypothetical protein	-
JQ467_RS24410 (JQ467_24385)	5243724..5244692	-	969	WP_124117653.1	hypothetical protein	-
JQ467_RS24415 (JQ467_24390)	5244689..5246056	-	1368	WP_124117655.1	ATP-binding protein	-
JQ467_RS24420 (JQ467_24395)	5246189..5247295	+	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ467_RS24425 (JQ467_24400)	5247624..5247929	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ467_RS24430 (JQ467_24405)	5247941..5248219	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ467_RS24435	5248272..5248400	-	129	Protein_4837	integrase	-
JQ467_RS24440 (JQ467_24410)	5248548..5250776	+	2229	WP_003099265.1	TonB-dependent receptor	-
JQ467_RS24445 (JQ467_24415)	5250846..5251493	-	648	WP_003095021.1	carbonate dehydratase	-
JQ467_RS24450 (JQ467_24420)	5251555..5252793	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190179 WP_003113526.1 NZ_CP069326:c5248219-5247941 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190179 NZ_CP091127:10722-11027 [Serratia marcescens]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190179 WP_003113527.1 NZ_CP069326:c5247929-5247624 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190179 NZ_CP091127:10502-10720 [Serratia marcescens]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3