Detailed information of TA system TA190169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1501360..1501955
• Replicon: chromosome
• Accession: NZ_CP069325
• Organism: Pseudomonas aeruginosa strain R02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ458_RS06945
• Protein ID: WP_003113526.1
• Coordinates: 1501360..1501638 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ458_RS06950
• Protein ID: WP_003113527.1
• Coordinates: 1501650..1501955 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ458_RS06925 (JQ458_06920)	1496786..1498024	+	1239	WP_010793756.1	C69 family dipeptidase	-
JQ458_RS06930 (JQ458_06925)	1498086..1498733	+	648	WP_003095021.1	carbonate dehydratase	-
JQ458_RS06935 (JQ458_06930)	1498803..1501031	-	2229	WP_124120912.1	TonB-dependent receptor	-
JQ458_RS06940	1501179..1501307	+	129	Protein_1368	integrase	-
JQ458_RS06945 (JQ458_06935)	1501360..1501638	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ458_RS06950 (JQ458_06940)	1501650..1501955	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ458_RS06955 (JQ458_06945)	1502284..1503390	-	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ458_RS06960 (JQ458_06950)	1503523..1504890	+	1368	WP_124117655.1	ATP-binding protein	-
JQ458_RS06965 (JQ458_06955)	1504887..1505855	+	969	WP_124117653.1	hypothetical protein	-
JQ458_RS06970 (JQ458_06960)	1505887..1506222	-	336	WP_124117651.1	hypothetical protein	-
JQ458_RS06975 (JQ458_06965)	1506278..1506544	-	267	WP_023088595.1	hypothetical protein	-
JQ458_RS06980 (JQ458_06970)	1506654..1506926	+	273	WP_124117649.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190169 WP_003113526.1 NZ_CP069325:1501360-1501638 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190169 NZ_CP091125:1287920-1288174 [Serratia marcescens]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190169 WP_003113527.1 NZ_CP069325:1501650-1501955 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190169 NZ_CP091125:1288171-1288515 [Serratia marcescens]
ATGATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGA
GTTTGTCGGGCTGAATGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTTAAGAAAGAAGGCGCGATCTCACTCG
CGGTCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCG
CCGGAACGCCATCAGGCGTTAGCCATTGCCGCACAGGCCACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGA
TCTGGTTATCGAAAAGCATTCCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3