Detailed information of TA system TA190167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5233200..5233795
• Replicon: chromosome
• Accession: NZ_CP069324
• Organism: Pseudomonas aeruginosa strain R05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ461_RS24350
• Protein ID: WP_003113526.1
• Coordinates: 5233517..5233795 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ461_RS24345
• Protein ID: WP_003113527.1
• Coordinates: 5233200..5233505 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ461_RS24325 (JQ461_24300)	5229276..5229533	+	258	WP_010793759.1	ATP-binding protein	-
JQ461_RS24330 (JQ461_24305)	5229909..5230772	-	864	WP_010793758.1	integrase domain-containing protein	-
JQ461_RS24335 (JQ461_24310)	5231374..5232516	-	1143	WP_010793757.1	STY4528 family pathogenicity island replication protein	-
JQ461_RS24345 (JQ461_24320)	5233200..5233505	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ461_RS24350 (JQ461_24325)	5233517..5233795	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ461_RS24355	5233848..5233976	-	129	Protein_4820	integrase	-
JQ461_RS24360 (JQ461_24330)	5234124..5236352	+	2229	WP_003099265.1	TonB-dependent receptor	-
JQ461_RS24365 (JQ461_24335)	5236422..5237069	-	648	WP_003095021.1	carbonate dehydratase	-
JQ461_RS24370 (JQ461_24340)	5237131..5238369	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190167 WP_003113526.1 NZ_CP069324:c5233795-5233517 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190167 NZ_CP091125:c595351-595148 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCGGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190167 WP_003113527.1 NZ_CP069324:c5233505-5233200 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190167 NZ_CP091125:c595770-595402 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
GACCTTGGGAGACAGCCACCACGGGTGGGTCAACGATCCGACATCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCAGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACTTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAGACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: V6ALY3