Detailed information of TA system TA190161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5247169..5247764
• Replicon: chromosome
• Accession: NZ_CP069323
• Organism: Pseudomonas aeruginosa strain R06

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JQ462_RS24430
• Protein ID: WP_003113526.1
• Coordinates: 5247486..5247764 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQ462_RS24425
• Protein ID: WP_003113527.1
• Coordinates: 5247169..5247474 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ462_RS24395 (JQ462_24365)	5242198..5242470	-	273	WP_124117649.1	DNA-binding protein	-
JQ462_RS24400 (JQ462_24370)	5242580..5242846	+	267	WP_023088595.1	hypothetical protein	-
JQ462_RS24405 (JQ462_24375)	5242902..5243237	+	336	WP_124117651.1	hypothetical protein	-
JQ462_RS24410 (JQ462_24380)	5243269..5244237	-	969	WP_124117653.1	hypothetical protein	-
JQ462_RS24415 (JQ462_24385)	5244234..5245601	-	1368	WP_124117655.1	ATP-binding protein	-
JQ462_RS24420 (JQ462_24390)	5245734..5246840	+	1107	WP_124117657.1	DNA cytosine methyltransferase	-
JQ462_RS24425 (JQ462_24395)	5247169..5247474	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
JQ462_RS24430 (JQ462_24400)	5247486..5247764	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQ462_RS24435	5247817..5247945	-	129	Protein_4838	integrase	-
JQ462_RS24440 (JQ462_24405)	5248093..5250321	+	2229	WP_003099265.1	TonB-dependent receptor	-
JQ462_RS24445 (JQ462_24410)	5250391..5251038	-	648	WP_003095021.1	carbonate dehydratase	-
JQ462_RS24450 (JQ462_24415)	5251100..5252338	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T190161 WP_003113526.1 NZ_CP069323:c5247764-5247486 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190161 NZ_CP091123:4220903-4221247 [Serratia marcescens]
ATGTGGCAAGTGGTGACCGTCGAACGGTTCGACGACTGGTTTTTAGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTTCAAATTACAGACGTTCGGCCCGCAATTGGCACGGCCGCATGCCGATACGCTGCATTTTTCCGACGCGGTCC
GGCAGTTGAAGGAGCTGCGCATTCAACATCGCGGGCGCCCTTTCAGGGTATTTTTCGCCTTTGACCCACAGCGGCAAGCG
GTATTGCTGTGCGGCGGCGATAAAACCGGCGACCAACGCTTTTACCAGCGCATGTTGCCCATCGCCGCCATGGAGTTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190161 WP_003113527.1 NZ_CP069323:c5247474-5247169 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190161 NZ_CP091123:4221253-4221555 [Serratia marcescens]
ATGGCCAAACCTTTATCCCAACTGATCGACAAGCTCGATCCCGCCGTCGTTGCCACTGCCAGGCAAAAAGCGGACAAGGA
AATTTTCGAGCTGCGGCTGGCGATGCTGAGGGAAGAGCTGGCGGTCTCTCAGGTTGAATTGGCGAAGCGGCTCGGCATCA
GCCAACCCTCCGTCGCCAATTTGGAAAAGCGCGGCAGCGAAATCAAACTGTCATCGCTCAAACGCTATATCGAGGCGATG
GGTGGCACGCTGTCGCTGGATGTGCAATTGCCCAATGGCCAGCACCGCCGCATGACGCTGTAA

Structures

Toxin

• AlphaFold DB: V6ALY3