Detailed information of TA system TA190130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 289583..290206
• Replicon: chromosome
• Accession: NZ_CP069318
• Organism: Delftia lacustris strain R54

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1I1B5B2
• Locus tag: JQN63_RS01270
• Protein ID: WP_016448670.1
• Coordinates: 289583..289864 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQN63_RS01275
• Protein ID: WP_013800141.1
• Coordinates: 289907..290206 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQN63_RS01245	284939..285802	+	864	WP_016448674.1	branched-chain amino acid ABC transporter permease	-
JQN63_RS01250	285803..286777	+	975	WP_046238969.1	branched-chain amino acid ABC transporter permease	-
JQN63_RS01255	286764..287528	+	765	WP_203400259.1	ABC transporter ATP-binding protein	-
JQN63_RS01260	287515..288222	+	708	WP_016448672.1	ABC transporter ATP-binding protein	-
JQN63_RS01265	288278..289453	+	1176	WP_016452145.1	ABC transporter substrate-binding protein	-
JQN63_RS01270	289583..289864	+	282	WP_016448670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQN63_RS01275	289907..290206	+	300	WP_013800141.1	HigA family addiction module antidote protein	Antitoxin
JQN63_RS01280	290299..291072	-	774	WP_016448669.1	SDR family oxidoreductase	-
JQN63_RS01285	291218..292024	-	807	WP_016448668.1	IclR family transcriptional regulator	-
JQN63_RS01290	292069..292842	-	774	WP_016448667.1	ferredoxin--NADP reductase	-
JQN63_RS01295	293104..294039	-	936	WP_016448666.1	LysR family transcriptional regulator	-
JQN63_RS01300	294133..294924	+	792	WP_016448665.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11057.71 Da
• Isoelectric Point: 10.1057

>T190130 WP_016448670.1 NZ_CP069318:289583-289864 [Delftia lacustris]
MTIQSFKCPDTEQLFQQRRVRRWSSIEVVALRKLRMLHAAHVLQDLRVPPGNRLEALQGDRKGQHSIRINDQWRVCFVWT
HEGPSQLEIVDYH

Nucleotide

• Length: 282 bp

>T190130 NZ_CP091120:4157447-4157833 [Serratia marcescens]
ATGGACATTTTCGTCACGGATGATTTCGACAAATTTATGAAGAAAAATCGCATCGTCGATCAAAAGATTTGCACCGCGGC
TCACGAACTCGCACACGGTAACCATGACGGCGATCTGGGCGGCGGCGTCTACAAGAAACGTATTTCGCTCAATCGGGGTA
AACGGGGTGGTGCTCGATCCATCGTGGCGTTTAAACGCGGTCGTCATCAGTATTTTATTGATGGATGGCTGAAAAACACC
GTAAAACAGAACGGTGCAAAAGAAATCAACGATGATGAATTGGCAACCTATCGCGAGCTCGCCAGAGATTTTCTTGCCAT
GCCGCCGGAAATTATCAAACGAGCCATCGATAGCGGCTACTTGCGAGAGGTGAAATGCGATGACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10990.57 Da
• Isoelectric Point: 4.5183

>AT190130 WP_013800141.1 NZ_CP069318:289907-290206 [Delftia lacustris]
MNKPLNLPTPGEILATEFLEPMGLTQYRLAQAIHVPQTRIAAIVKNGRSITADTALRLARFFGTSVEFWMNLQSQYDADI
AREAIGDDLESIEPFALQA

Nucleotide

• Length: 300 bp

>AT190130 NZ_CP091120:4157826-4158170 [Serratia marcescens]
ATGACTGATAATCGCCTGCTCAGACTGCAAAAAATGGCACAGCGTTTTAATGAGATCGGTGCGGTGTCAGACGATACCGT
ACGCAACATCGCCACACGAGTACAGGCGCAGGAACAACATGCCCAGCGTGAGCAGCATGATCAAATGGACGGCGCACGCA
TCAAAATGCTGCGCAGACGGCTCGGCTTGAGCCAGGCCGATTTAGCGGCCGTGGTCAACATGTCGACCACCAGCGTACAA
AAATGGGAACGTAACGCGGTAAAACCCCAGGGAGCCGCACTCAGAATGCTGGAAATTATCGAACAGAAAGGGGTCGCCAG
CCTGATCATCTCACCACGAAACTGA

Structures

Toxin

• AlphaFold DB: A0A1I1B5B2