Detailed information of TA system TA190124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1021557..1022118
• Replicon: chromosome
• Accession: NZ_CP069308
• Organism: Glaesserella parasuis strain EHP1804

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143CE91
• Locus tag: JQC68_RS05265
• Protein ID: WP_005712614.1
• Coordinates: 1021840..1022118 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: JQC68_RS05260
• Protein ID: WP_005712616.1
• Coordinates: 1021557..1021829 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQC68_RS05235	1017133..1018017	+	885	WP_021115963.1	LysR family transcriptional regulator	-
JQC68_RS05240	1018177..1018460	-	284	Protein_1016	transposase	-
JQC68_RS05245	1018451..1018651	-	201	WP_021115966.1	hypothetical protein	-
JQC68_RS05250	1018776..1020263	-	1488	WP_160586050.1	DUF853 domain-containing protein	-
JQC68_RS05255	1020273..1021493	-	1221	WP_035494018.1	ATP-dependent RNA helicase RhlB	-
JQC68_RS05260	1021557..1021829	-	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
JQC68_RS05265	1021840..1022118	-	279	WP_005712614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQC68_RS05270	1022267..1023277	+	1011	WP_038513972.1	ornithine carbamoyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10326.71 Da
• Isoelectric Point: 10.0505

>T190124 WP_005712614.1 NZ_CP069308:c1022118-1021840 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T190124 NZ_CP091120:c1421637-1421332 [Serratia marcescens]
ATGCCCGCGTTTCGCCTCACCCCGCAGGCCCAACAAGATCTGCTGGCTATTCGTCATTTCACCATCGAGCACTGGGGCCA
AGCGCAATCTCGCCGCTATCTTGAGCAGTTGAGAGAGGTCATGCATCATTTGGCAGATATGCCGGAAGCGGGTAAAGCTC
ACTTCCACGATCTGGGCGAAGAGATACGCAGTTTTCCTTACGCCAGCCATAGGATCTACTACCGCAACCGCCCTGCCGGC
ATCACCGTTCTCGCCATTCTGCATCAGGCGATGGTGCCTCACCGGCATCTGGAACAACGGCTGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT190124 WP_005712616.1 NZ_CP069308:c1021829-1021557 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT190124 NZ_CP091120:c1421899-1421639 [Serratia marcescens]
ATGTACGCACTCACCGCGAACGAGGCCAAAACCCAATTCGGCGATTTGCTGCTCAAGGCGCAGCGTGAACCGGTGCAAAT
CAATCGCAACGGCAAACCGGTCGCCGTCGTCATGTCTGTAGAAGATTACCAGGCGCTGGAAGATCTCAAATTAAGCGCAC
TGCAGCAAAAAATCGCGCAGGCTACCGCCGAGGAAGACGCCGGTAAGGCGGTAGACGGCGATGCCTTCTTCAACCGTCTG
CTCACCGATGAAACAGACTGA

Structures

Toxin

• AlphaFold DB: A0A143CE91