Detailed information of TA system TA190110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1136769..1137421
• Replicon: plasmid unnamed
• Accession: NZ_CP069246
• Organism: Ralstonia pseudosolanacearum strain PeaFJ1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: JNO62_RS22310
• Protein ID: WP_028861315.1
• Coordinates: 1136769..1137113 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNO62_RS22315
• Protein ID: WP_020425351.1
• Coordinates: 1137110..1137421 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNO62_RS22295 (JNO62_22155)	1132464..1133627	-	1164	WP_011003438.1	2-methylcitrate synthase	-
JNO62_RS22300 (JNO62_22160)	1133679..1134575	-	897	WP_071092368.1	methylisocitrate lyase	-
JNO62_RS22305 (JNO62_22165)	1134769..1136718	+	1950	WP_111375053.1	propionate catabolism operon regulatory protein PrpR	-
JNO62_RS22310 (JNO62_22170)	1136769..1137113	+	345	WP_028861315.1	hypothetical protein	Toxin
JNO62_RS22315 (JNO62_22175)	1137110..1137421	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
JNO62_RS22320 (JNO62_22180)	1137561..1138370	-	810	WP_043885575.1	DUF2242 domain-containing protein	-
JNO62_RS22325 (JNO62_22185)	1139029..1139688	-	660	WP_127592217.1	hypothetical protein	-
JNO62_RS22330 (JNO62_22190)	1140016..1141854	+	1839	WP_080624796.1	3'-5' exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / adeG / cheY / cheB / cheW / motA / cyaB / flgC / flgG / fliM / fliG / fliI / acrB	1..2008534	2008534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T190110 WP_028861315.1 NZ_CP069246:1136769-1137113 [Ralstonia pseudosolanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T190110 NZ_CP091098:c129606-129319 [Pantoea agglomerans]
GTGAGTTATACCGTCAAATTCAGGGAAGAGGCATTTAAAGAATGGCAGAAGCTGGACAAATCTCTTCAGCAGCAGTTTGC
CAAAAAACTGAAGAAATGTTGTGACAATCCCCATATTCCTTCTGCCAGACTACGTGGGATTAAAGACTGCTATAAAATCA
AACTCAGAGCGTCCGGGTTTCGCCTGGTTTATCAGGTAATAGACGATCAGCTGGTGATTGCCGTTGTTGCTGTGGGTAAA
CGCGAGCGCAGCGATGTTTATCATCTTGCCAGTGAGAGGATGCGGTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT190110 WP_020425351.1 NZ_CP069246:1137110-1137421 [Ralstonia pseudosolanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT190110 NZ_CP091098:c129854-129603 [Pantoea agglomerans]
ATGCCAAACATTATTTTAAGCGATACCAGTGCCAGCGTAAGCGAACTGAAGAAAAACCCGATGGCCACGGTTAGTGCAGG
TGACGGTTATCCTGTCGCCATTCTTAACCGCAACCAGCCTGCATTCTATTGCGTCCCGGCTGAGCTTTATGAACAAATGC
TTGATGCCCTTGACGATCAGGAACTGGTTAAGCTGGTCAGTGAACGCAGCGGCCAGCGTCTGCTTGATGTCGATTTGGAT
GCGCTTTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4