Detailed information of TA system TA190048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2272406..2273087
• Replicon: chromosome
• Accession: NZ_CP069177
• Organism: Pseudomonas aeruginosa strain Z154

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: IS492_RS10625
• Protein ID: WP_003111825.1
• Coordinates: 2272406..2272771 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS492_RS10630
• Protein ID: WP_003145733.1
• Coordinates: 2272764..2273087 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS492_RS10595 (IS492_10585)	2268402..2268836	-	435	WP_003158601.1	RidA family protein	-
IS492_RS10600 (IS492_10590)	2269057..2270010	+	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
IS492_RS10605 (IS492_10595)	2269983..2270867	-	885	WP_003158600.1	LysR family transcriptional regulator	-
IS492_RS10610 (IS492_10600)	2270968..2271393	+	426	WP_003114206.1	VOC family protein	-
IS492_RS10615 (IS492_10605)	2271424..2271696	-	273	WP_023090041.1	hypothetical protein	-
IS492_RS10620 (IS492_10610)	2271903..2272142	+	240	WP_044060930.1	hypothetical protein	-
IS492_RS10625 (IS492_10615)	2272406..2272771	+	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS492_RS10630 (IS492_10620)	2272764..2273087	+	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
IS492_RS10635 (IS492_10625)	2273451..2274539	-	1089	WP_031652783.1	DUF3396 domain-containing protein	-
IS492_RS10640 (IS492_10630)	2274556..2275257	-	702	WP_031652782.1	VRR-NUC domain-containing protein	-
IS492_RS10645 (IS492_10635)	2275254..2275772	-	519	WP_031652781.1	PAAR domain-containing protein	-
IS492_RS10650 (IS492_10640)	2275947..2276282	-	336	WP_003140884.1	TM2 domain-containing protein	-
IS492_RS10655 (IS492_10645)	2276523..2277161	-	639	WP_003119439.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T190048 WP_003111825.1 NZ_CP069177:2272406-2272771 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T190048 NZ_CP091066:c784615-784427 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT190048 WP_003145733.1 NZ_CP069177:2272764-2273087 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT190048 NZ_CP091066:c784904-784644 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACAACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATTACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCGATT
TTTTATAGGTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: V6AKP0