Detailed information of TA system TA190046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1530003..1530598
• Replicon: chromosome
• Accession: NZ_CP069177
• Organism: Pseudomonas aeruginosa strain Z154

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: IS492_RS07085
• Protein ID: WP_003117425.1
• Coordinates: 1530003..1530281 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS492_RS07090
• Protein ID: WP_003113527.1
• Coordinates: 1530293..1530598 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS492_RS07065 (IS492_07065)	1525428..1526666	+	1239	WP_031652874.1	C69 family dipeptidase	-
IS492_RS07070 (IS492_07070)	1526728..1527375	+	648	WP_003095021.1	carbonate dehydratase	-
IS492_RS07075 (IS492_07075)	1527445..1529673	-	2229	WP_003099265.1	TonB-dependent receptor	-
IS492_RS07080	1529822..1529950	+	129	Protein_1396	integrase	-
IS492_RS07085 (IS492_07080)	1530003..1530281	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS492_RS07090 (IS492_07085)	1530293..1530598	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
IS492_RS07100 (IS492_07095)	1531004..1532104	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
IS492_RS07105 (IS492_07100)	1532145..1532729	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
IS492_RS07110 (IS492_07105)	1532771..1533385	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
IS492_RS07115 (IS492_07110)	1533502..1534443	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
IS492_RS07125 (IS492_07120)	1534610..1535458	-	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T190046 WP_003117425.1 NZ_CP069177:1530003-1530281 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T190046 NZ_CP091065:c4523040-4522783 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT190046 WP_003113527.1 NZ_CP069177:1530293-1530598 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT190046 NZ_CP091065:c4522771-4522226 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5