Detailed information of TA system TA190038

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128019..128445
• Replicon: plasmid pB
• Accession: NZ_CP069172
• Organism: Klebsiella pneumoniae strain KPWX136

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLH18_RS28865
• Protein ID: WP_001312861.1
• Coordinates: 128019..128177 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	128221..128445 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH18_RS28835	123287..123991	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JLH18_RS28840	124272..124655	-	384	WP_000124981.1	relaxosome protein TraM	-
JLH18_RS28845	124932..125579	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
JLH18_RS28850	125876..126697	-	822	WP_001234445.1	DUF945 domain-containing protein	-
JLH18_RS28855	126808..127104	-	297	WP_001272251.1	hypothetical protein	-
JLH18_RS28860	127404..127781	+	378	Protein_160	hypothetical protein	-
JLH18_RS28865	128019..128177	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	128221..128445	-	225	NuclAT_0	-	Antitoxin
-	128221..128445	-	225	NuclAT_0	-	Antitoxin
-	128221..128445	-	225	NuclAT_0	-	Antitoxin
-	128221..128445	-	225	NuclAT_0	-	Antitoxin
JLH18_RS28870	128457..129176	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
JLH18_RS28875	129173..129607	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JLH18_RS28880	129676..131699	-	2024	Protein_164	ParB/RepB/Spo0J family partition protein	-
JLH18_RS28885	131760..131993	-	234	WP_000006003.1	DUF905 family protein	-
JLH18_RS28890	132051..132578	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 / blaSHV-12	-	1..134972	134972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190038 WP_001312861.1 NZ_CP069172:c128177-128019 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190038 NZ_CP091065:2429846-2429953 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT190038 NZ_CP069172:c128445-128221 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin