Detailed information of TA system TA190034

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29208..29461
• Replicon: plasmid pB
• Accession: NZ_CP069172
• Organism: Klebsiella pneumoniae strain KPWX136

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JLH18_RS28260
• Protein ID: WP_001312851.1
• Coordinates: 29208..29357 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	29402..29461 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH18_RS28225	24567..24982	-	416	Protein_33	IS1 family transposase	-
JLH18_RS28230	25231..25632	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JLH18_RS28235	25565..25822	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JLH18_RS28240	25915..26568	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JLH18_RS28245	27507..28364	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
JLH18_RS28250	28357..28431	-	75	WP_001375168.1	RepA leader peptide Tap	-
JLH18_RS28255	28676..28924	-	249	WP_000083837.1	replication regulatory protein RepA	-
JLH18_RS28260	29208..29357	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	29402..29461	+	60	NuclAT_1	-	Antitoxin
-	29402..29461	+	60	NuclAT_1	-	Antitoxin
-	29402..29461	+	60	NuclAT_1	-	Antitoxin
-	29402..29461	+	60	NuclAT_1	-	Antitoxin
JLH18_RS28265	29662..29892	-	231	WP_001736714.1	hypothetical protein	-
JLH18_RS28270	30056..30655	-	600	WP_032083981.1	hypothetical protein	-
JLH18_RS28275	31041..31241	-	201	WP_015059022.1	hypothetical protein	-
JLH18_RS28280	31373..31933	-	561	WP_000139328.1	fertility inhibition protein FinO	-
JLH18_RS28285	31988..32734	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JLH18_RS28290	32754..32915	-	162	Protein_46	DNA helicase	-
JLH18_RS28295	32979..33683	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JLH18_RS28300	33735..34079	+	345	Protein_48	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 / blaSHV-12	-	1..134972	134972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T190034 WP_001312851.1 NZ_CP069172:c29357-29208 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T190034 NZ_CP091065:2055222-2055509 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT190034 NZ_CP069172:29402-29461 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin