Detailed information of TA system TA190030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31066..31793
• Replicon: plasmid pA
• Accession: NZ_CP069171
• Organism: Klebsiella pneumoniae strain KPWX136

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: JLH18_RS27170
• Protein ID: WP_011251285.1
• Coordinates: 31066..31377 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLH18_RS27175
• Protein ID: WP_011251286.1
• Coordinates: 31374..31793 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH18_RS27150	26585..27079	-	495	WP_004212794.1	thermonuclease family protein	-
JLH18_RS27155	27257..28206	+	950	Protein_29	DUF1173 family protein	-
JLH18_RS27160	29592..30289	-	698	Protein_30	IS1 family transposase	-
JLH18_RS27165	30403..30861	+	459	Protein_31	DDE-type integrase/transposase/recombinase	-
JLH18_RS27170	31066..31377	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
JLH18_RS27175	31374..31793	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
JLH18_RS27180	31940..32908	+	969	WP_074428168.1	IS5 family transposase	-
JLH18_RS27185	33010..33345	-	336	Protein_35	conjugal transfer protein TraI	-
JLH18_RS27190	33359..34147	-	789	WP_040217257.1	hypothetical protein	-
JLH18_RS27195	34168..34611	-	444	WP_048333569.1	hypothetical protein	-
JLH18_RS27200	35207..35962	+	756	Protein_38	hypothetical protein	-
JLH18_RS27205	36155..36625	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / iroN	1..191359	191359
	-	inside	IScluster/Tn	-	-	24399..49422	25023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T190030 WP_011251285.1 NZ_CP069171:31066-31377 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T190030 NZ_CP091065:1611918-1612169 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT190030 WP_011251286.1 NZ_CP069171:31374-31793 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT190030 NZ_CP091065:1612166-1612420 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1