Detailed information of TA system TA189995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2323697..2324259
• Replicon: chromosome
• Accession: NZ_CP069159
• Organism: Citrobacter sp. R56

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JM656_RS11035
• Protein ID: WP_203361275.1
• Coordinates: 2323978..2324259 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JM656_RS11030
• Protein ID: WP_203361179.1
• Coordinates: 2323697..2323981 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JM656_RS11015	2319042..2322089	+	3048	WP_203361178.1	formate dehydrogenase-N subunit alpha	-
JM656_RS11020	2322103..2322987	+	885	WP_192612223.1	formate dehydrogenase N subunit beta	-
JM656_RS11025	2322980..2323633	+	654	WP_162382270.1	formate dehydrogenase-N subunit gamma	-
JM656_RS11030	2323697..2323981	-	285	WP_203361179.1	HigA family addiction module antidote protein	Antitoxin
JM656_RS11035	2323978..2324259	-	282	WP_203361275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JM656_RS11040	2324445..2325455	-	1011	WP_203361180.1	alcohol dehydrogenase AdhP	-
JM656_RS11045	2325694..2327412	-	1719	WP_203361181.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10690.16 Da
• Isoelectric Point: 7.4728

>T189995 WP_203361275.1 NZ_CP069159:c2324259-2323978 [Citrobacter sp. R56]
MIMSFRHKGLRDLFLHGRTSGVLTTQVKRLRHRLAVIDAACHINDIDMPGYRLHSLSGDRDGVWAISVSGNWRITFEFVN
GDAYILDYEDYHA

Nucleotide

• Length: 282 bp

>T189995 NZ_CP091056:c1401058-1400633 [Ornithinimicrobium sp. INDO-MA30-4]
ATGATCGTCCTCGACACTAACGTCATCTCGGAGATCTTTCGGCCCTCTCCGGAGCCTCGTGTCGTCGAGTGGCTTGTGTC
CTTGACGGGCGACGTCGCGATCACTTCCATCACTCTCGCGGAGTTGCTTGCCGGTGTGCGCAGGCTCCCGAACGGCCAAC
GCAAAGACGAGCTGGCGCAGAGGATCGATGAAGCGGTAGCGCCGTATCGGGGGAGCCGGTCGGTGCTCGCATTCGATGCT
GACGCGGCGGAGCGATACGCGGAGGTGCTCGCGTCGCGTGAGGCAGCAGGCGCACCGGTCAGTACCGCCGACGCCCAGAT
CGCCGCGATCTGCCTGGCGCACGGGGCCACCTGTGCTACGCGCAATGTGAAGGACTTCCAGCACACCGGCGTCGAACTGG
TGGACCCATGGAAGGTGGACGCGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10368.05 Da
• Isoelectric Point: 8.8777

>AT189995 WP_203361179.1 NZ_CP069159:c2323981-2323697 [Citrobacter sp. R56]
MKMANHPRPGEIIQEALNELNVSLREFARAMDIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQTAWSLTEAE
KTVDISRLRHLATQ

Nucleotide

• Length: 285 bp

>AT189995 NZ_CP091056:c1401288-1401055 [Ornithinimicrobium sp. INDO-MA30-4]
ATGTCATCCATCATCGTGCGCGGTCTCGATGACCACGTGAAGCAGCAGCTCTCATCGCAGGCGAAGGAGCACGGGCGGTC
AATGGAGGCCGAGGTTCGCGACATCCTCACGAAGGCCGCTCGACGGCCGCACATCGGCATGGCTCTTCTGTCTGCGGCAC
GGGATGTCGATGGGGTCGACGAGTTGCCGATTCCGACGCGCGATGATGTCGCAAGGGCGGCGGACCTCGAATGA