Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2082688..2083190 Replicon chromosome
Accession NZ_CP069132
Organism Escherichia coli BW25113 substr. CHXR1G20

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JQK35_RS10310 Protein ID WP_000767829.1
Coordinates 2082688..2082942 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JQK35_RS10315 Protein ID WP_001259255.1
Coordinates 2082939..2083190 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQK35_RS10280 2077703..2077930 - 228 WP_000234896.1 sulfurtransferase TusA -
JQK35_RS10285 2077944..2079002 - 1059 WP_000492339.1 transport protein YeeE -
JQK35_RS10290 2079181..2080539 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JQK35_RS10295 2080529..2080591 - 63 WP_010723108.1 membrane protein YoeI -
JQK35_RS10300 2080806..2081735 - 930 WP_000803351.1 LysR family transcriptional regulator -
JQK35_RS10305 2081781..2082605 - 825 WP_000754737.1 SDR family oxidoreductase -
JQK35_RS10310 2082688..2082942 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JQK35_RS10315 2082939..2083190 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JQK35_RS10320 2083473..2083523 + 51 WP_001364200.1 his operon leader peptide -
JQK35_RS10325 2083669..2084568 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
JQK35_RS10330 2084574..2085878 + 1305 WP_000009594.1 histidinol dehydrogenase -
JQK35_RS10335 2085875..2086945 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
JQK35_RS10340 2086945..2088012 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T189929 WP_000767829.1 NZ_CP069132:c2082942-2082688 [Escherichia coli BW25113]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T189929 NZ_CP091027:c88398-88249 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT189929 WP_001259255.1 NZ_CP069132:c2083190-2082939 [Escherichia coli BW25113]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT189929 NZ_CP091027:88442-88503 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References