Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 242444..242985 Replicon chromosome
Accession NZ_CP069132
Organism Escherichia coli BW25113 substr. CHXR1G20

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JQK35_RS01130 Protein ID WP_000615983.1
Coordinates 242444..242722 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JQK35_RS01135 Protein ID WP_000729703.1
Coordinates 242725..242985 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQK35_RS01115 240026..240604 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
JQK35_RS01120 240810..241577 + 768 WP_000333380.1 class II glutamine amidotransferase -
JQK35_RS01125 241548..242288 - 741 WP_001225679.1 murein L,D-transpeptidase -
JQK35_RS01130 242444..242722 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JQK35_RS01135 242725..242985 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JQK35_RS01140 243195..243944 + 750 WP_000056849.1 C40 family peptidase -
JQK35_RS01145 244120..244617 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JQK35_RS01150 244841..246553 - 1713 Protein_222 flagellar biosynthesis protein FlhA -
JQK35_RS01155 246525..247310 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T189903 WP_000615983.1 NZ_CP069132:c242722-242444 [Escherichia coli BW25113]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T189903 NZ_CP091024:c4558383-4558210 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT189903 WP_000729703.1 NZ_CP069132:c242985-242725 [Escherichia coli BW25113]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT189903 NZ_CP091024:c4558707-4558411 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References