Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3447256..3447842 Replicon chromosome
Accession NZ_CP069080
Organism Pseudomonas putida strain LWPZF

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag JNO42_RS15985 Protein ID WP_239606744.1
Coordinates 3447256..3447534 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag JNO42_RS15990 Protein ID WP_003252274.1
Coordinates 3447543..3447842 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNO42_RS15975 (JNO42_15870) 3444056..3445252 + 1197 WP_239606743.1 succinyldiaminopimelate transaminase -
JNO42_RS15980 (JNO42_15875) 3445358..3447004 - 1647 WP_012053546.1 Na+/H+ antiporter -
JNO42_RS15985 (JNO42_15880) 3447256..3447534 + 279 WP_239606744.1 type II toxin-antitoxin system toxin GraT Toxin
JNO42_RS15990 (JNO42_15885) 3447543..3447842 + 300 WP_003252274.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
JNO42_RS15995 (JNO42_15890) 3447967..3448314 + 348 WP_003252272.1 ArsC family reductase -
JNO42_RS16000 (JNO42_15895) 3448346..3449380 + 1035 WP_004574732.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
JNO42_RS16005 (JNO42_15900) 3449462..3450667 + 1206 WP_239606745.1 cysteine desulfurase -
JNO42_RS16010 (JNO42_15905) 3450664..3451074 + 411 WP_003252266.1 SufE family protein -
JNO42_RS16015 (JNO42_15910) 3451183..3451992 - 810 WP_003252264.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(12-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-90)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10439.73 Da        Isoelectric Point: 7.2448

>T189889 WP_239606744.1 NZ_CP069080:3447256-3447534 [Pseudomonas putida]
MIQSISCADTEALFTTGKTRRWSDIKSVAERKLAMPDAATELRDLRSPPGNRLESLSGNRAGQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

>T189889 NZ_CP091024:933335-933742 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10891.52 Da        Isoelectric Point: 5.6714

>AT189889 WP_003252274.1 NZ_CP069080:3447543-3447842 [Pseudomonas putida]
MLKNGMRPIHPGEILREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp

>AT189889 NZ_CP091024:933088-933354 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References