Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3544508..3545178 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag IMW72_RS16790 Protein ID WP_003899954.1
Coordinates 3544508..3544852 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag IMW72_RS16795 Protein ID WP_003899955.1
Coordinates 3544849..3545178 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS16755 3539581..3540441 + 861 WP_003416628.1 alpha/beta hydrolase -
IMW72_RS16760 3540416..3540931 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
IMW72_RS16765 3540947..3541158 + 212 Protein_3311 (R)-hydratase -
IMW72_RS16770 3541171..3541464 + 294 WP_003416635.1 hypothetical protein -
IMW72_RS16775 3541752..3543041 + 1290 WP_003416640.1 ATP-binding protein -
IMW72_RS16780 3543388..3543822 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
IMW72_RS16785 3543825..3544277 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
IMW72_RS16790 3544508..3544852 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IMW72_RS16795 3544849..3545178 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
IMW72_RS16805 3547074..3547421 + 348 WP_003899956.1 DUF2384 domain-containing protein -
IMW72_RS16810 3547418..3548038 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189879 WP_003899954.1 NZ_CP069078:3544508-3544852 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189879 NZ_CP091023:c3957-3679 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189879 WP_003899955.1 NZ_CP069078:3544849-3545178 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189879 NZ_CP091023:c4226-3957 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACAACAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACATCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References