Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2268673..2269592 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag IMW72_RS10670 Protein ID WP_003900449.1
Coordinates 2268987..2269592 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag IMW72_RS10665 Protein ID WP_003410124.1
Coordinates 2268673..2268978 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS10635 2263684..2264940 - 1257 WP_003902247.1 HNH endonuclease signature motif containing protein -
IMW72_RS10640 2265294..2265869 + 576 WP_003410100.1 hypothetical protein -
IMW72_RS10645 2265866..2266906 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
IMW72_RS10650 2267148..2267867 + 720 WP_003410108.1 DUF433 domain-containing protein -
IMW72_RS10655 2267857..2268273 + 417 WP_003410114.1 hypothetical protein -
IMW72_RS10660 2268289..2268588 - 300 WP_003410120.1 hypothetical protein -
IMW72_RS10665 2268673..2268978 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
IMW72_RS10670 2268987..2269592 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IMW72_RS10675 2269617..2269976 - 360 WP_003410131.1 hypothetical protein -
IMW72_RS10680 2270136..2270726 - 591 WP_057145698.1 hypothetical protein -
IMW72_RS10685 2270765..2271001 - 237 WP_003410133.1 hypothetical protein -
IMW72_RS10690 2271208..2272104 - 897 WP_003410136.1 hypothetical protein -
IMW72_RS10695 2272288..2273718 - 1431 Protein_2112 helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2261254..2272104 10850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T189861 WP_003900449.1 NZ_CP069078:c2269592-2268987 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189861 NZ_CP091022:692017-692331 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189861 WP_003410124.1 NZ_CP069078:c2268978-2268673 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189861 NZ_CP091022:692328-692744 [Escherichia coli]
ATGATTACGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References