Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2209024..2209892 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag IMW72_RS10320 Protein ID WP_010886136.1
Coordinates 2209024..2209401 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag IMW72_RS10325 Protein ID WP_003409886.1
Coordinates 2209443..2209892 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS10270 2204813..2205265 - 453 WP_003899095.1 lipoprotein -
IMW72_RS10275 2205329..2205730 + 402 WP_003409869.1 hypothetical protein -
IMW72_RS10280 2205723..2205905 - 183 WP_003409870.1 hypothetical protein -
IMW72_RS10285 2206019..2206369 - 351 WP_003409871.1 hypothetical protein -
IMW72_RS10290 2206380..2207282 - 903 WP_003409874.1 hypothetical protein -
IMW72_RS10295 2207303..2207494 - 192 WP_003409876.1 hypothetical protein -
IMW72_RS10300 2207495..2207791 - 297 WP_003409877.1 hypothetical protein -
IMW72_RS10305 2208031..2208246 + 216 WP_003409878.1 antitoxin -
IMW72_RS10310 2208243..2208554 + 312 WP_003903693.1 type II toxin-antitoxin system VapC family toxin -
IMW72_RS10315 2208528..2209049 - 522 WP_003903694.1 hypothetical protein -
IMW72_RS10320 2209024..2209401 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
IMW72_RS10325 2209443..2209892 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IMW72_RS10330 2209889..2210434 + 546 WP_003409891.1 SecB-like chaperone -
IMW72_RS10335 2210323..2210937 - 615 WP_003901296.1 hypothetical protein -
IMW72_RS10340 2210986..2211282 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
IMW72_RS10345 2211279..2211530 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
IMW72_RS10350 2211517..2212011 + 495 WP_003899099.1 hypothetical protein -
IMW72_RS10355 2212171..2212578 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
IMW72_RS10360 2212582..2212854 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
IMW72_RS10365 2212887..2214107 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T189855 WP_010886136.1 NZ_CP069078:2209024-2209401 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T189855 NZ_CP091020:c4545316-4544966 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT189855 WP_003409886.1 NZ_CP069078:2209443-2209892 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT189855 NZ_CP091020:c4545561-4545310 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References