Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3556586..3557256 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN135_RS16790 Protein ID WP_003899954.1
Coordinates 3556586..3556930 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN135_RS16795 Protein ID WP_003899955.1
Coordinates 3556927..3557256 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS16755 3551659..3552519 + 861 WP_003416628.1 alpha/beta hydrolase -
JN135_RS16760 3552494..3553009 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JN135_RS16765 3553025..3553236 + 212 Protein_3311 (R)-hydratase -
JN135_RS16770 3553249..3553542 + 294 WP_003416635.1 hypothetical protein -
JN135_RS16775 3553830..3555119 + 1290 WP_003416640.1 ATP-binding protein -
JN135_RS16780 3555466..3555900 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN135_RS16785 3555903..3556355 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN135_RS16790 3556586..3556930 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN135_RS16795 3556927..3557256 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN135_RS16800 3557794..3558141 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN135_RS16805 3558138..3558758 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
JN135_RS16810 3558918..3560183 - 1266 WP_003909837.1 hypothetical protein -
JN135_RS16815 3560351..3560560 + 210 WP_003416778.1 hypothetical protein -
JN135_RS16820 3560807..3561841 - 1035 WP_003905011.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189818 WP_003899954.1 NZ_CP069077:3556586-3556930 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189818 NZ_CP091018:293753-294280 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189818 WP_003899955.1 NZ_CP069077:3556927-3557256 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189818 NZ_CP091018:293480-293746 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References