Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2283170..2284089 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JN135_RS10735 Protein ID WP_003900449.1
Coordinates 2283484..2284089 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN135_RS10730 Protein ID WP_003410124.1
Coordinates 2283170..2283475 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS10700 2278181..2279437 - 1257 WP_003902247.1 HNH endonuclease signature motif containing protein -
JN135_RS10705 2279791..2280366 + 576 WP_003410100.1 hypothetical protein -
JN135_RS10710 2280363..2281403 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JN135_RS10715 2281645..2282364 + 720 WP_003410108.1 DUF433 domain-containing protein -
JN135_RS10720 2282354..2282770 + 417 WP_003410114.1 hypothetical protein -
JN135_RS10725 2282786..2283085 - 300 WP_003410120.1 hypothetical protein -
JN135_RS10730 2283170..2283475 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN135_RS10735 2283484..2284089 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN135_RS10740 2284114..2284473 - 360 WP_003410131.1 hypothetical protein -
JN135_RS10745 2284633..2285286 - 654 WP_014324107.1 hypothetical protein -
JN135_RS10750 2285262..2285498 - 237 WP_003410133.1 hypothetical protein -
JN135_RS10755 2285705..2286601 - 897 WP_003904759.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T189801 WP_003900449.1 NZ_CP069077:c2284089-2283484 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189801 NZ_CP091016:995348-995755 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189801 WP_003410124.1 NZ_CP069077:c2283475-2283170 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189801 NZ_CP091016:995101-995367 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References