Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3559213..3559883 Replicon chromosome
Accession NZ_CP069075
Organism Mycobacterium tuberculosis strain N0004

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN138_RS16870 Protein ID WP_003899954.1
Coordinates 3559213..3559557 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN138_RS16875 Protein ID WP_003899955.1
Coordinates 3559554..3559883 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN138_RS16835 3554286..3555146 + 861 WP_003416628.1 alpha/beta hydrolase -
JN138_RS16840 3555121..3555636 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JN138_RS16845 3555652..3555863 + 212 Protein_3334 (R)-hydratase -
JN138_RS16850 3555876..3556169 + 294 WP_003416635.1 hypothetical protein -
JN138_RS16855 3556457..3557746 + 1290 WP_003416640.1 ATP-binding protein -
JN138_RS16860 3558093..3558527 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN138_RS16865 3558530..3558982 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN138_RS16870 3559213..3559557 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN138_RS16875 3559554..3559883 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN138_RS16880 3560421..3560768 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN138_RS16885 3560765..3561385 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
JN138_RS16890 3561545..3562810 - 1266 WP_003902423.1 hypothetical protein -
JN138_RS16895 3562978..3563187 + 210 WP_003416778.1 hypothetical protein -
JN138_RS16900 3563222..3563407 + 186 WP_015630737.1 hypothetical protein -
JN138_RS16905 3563434..3564468 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189697 WP_003899954.1 NZ_CP069075:3559213-3559557 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189697 NZ_CP090930:3881970-3882227 [Salmonella enterica subsp. enterica serovar Agona]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGTATAACCACCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACGGTGAAGATTTCACAGGGGTGTA
TTGTGCTAATGGCGGACGGTAACGAGGTGCAGAAGCTGTGCGAACAGCTTTATCAGGCTGAACGGGTGGTTAAGGGGTTG
AAGGATGTACTGGTTTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189697 WP_003899955.1 NZ_CP069075:3559554-3559883 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189697 NZ_CP090930:c3882191-3881813 [Salmonella enterica subsp. enterica serovar Agona]
TTCAGCCTGATAAAGCTGTTCGCACAGCTTCTGCACCTCGTTACCGTCCGCCATTAGCACAATACACCCCTGTGAAATCT
TCACCGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAG
TAACAGGTGGTTATACAGGTTTCGCTGTTGATGTGGCGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAA
TTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAGCGAGTATGGGCGCGACATCCTGGCACCGCGCC
CCCCTCTCCCCGGCCAGCACCGGGCGGTGATGAGCAACCGGCTACCGGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References