Detailed information of TA system TA189697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3559213..3559883
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: JN138_RS16870
• Protein ID: WP_003899954.1
• Coordinates: 3559213..3559557 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: JN138_RS16875
• Protein ID: WP_003899955.1
• Coordinates: 3559554..3559883 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS16835	3554286..3555146	+	861	WP_003416628.1	alpha/beta hydrolase	-
JN138_RS16840	3555121..3555636	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
JN138_RS16845	3555652..3555863	+	212	Protein_3334	(R)-hydratase	-
JN138_RS16850	3555876..3556169	+	294	WP_003416635.1	hypothetical protein	-
JN138_RS16855	3556457..3557746	+	1290	WP_003416640.1	ATP-binding protein	-
JN138_RS16860	3558093..3558527	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
JN138_RS16865	3558530..3558982	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JN138_RS16870	3559213..3559557	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN138_RS16875	3559554..3559883	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
JN138_RS16880	3560421..3560768	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
JN138_RS16885	3560765..3561385	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
JN138_RS16890	3561545..3562810	-	1266	WP_003902423.1	hypothetical protein	-
JN138_RS16895	3562978..3563187	+	210	WP_003416778.1	hypothetical protein	-
JN138_RS16900	3563222..3563407	+	186	WP_015630737.1	hypothetical protein	-
JN138_RS16905	3563434..3564468	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T189697 WP_003899954.1 NZ_CP069075:3559213-3559557 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T189697 NZ_CP090930:3881970-3882227 [Salmonella enterica subsp. enterica serovar Agona]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGTATAACCACCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACGGTGAAGATTTCACAGGGGTGTA
TTGTGCTAATGGCGGACGGTAACGAGGTGCAGAAGCTGTGCGAACAGCTTTATCAGGCTGAACGGGTGGTTAAGGGGTTG
AAGGATGTACTGGTTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT189697 WP_003899955.1 NZ_CP069075:3559554-3559883 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT189697 NZ_CP090930:c3882191-3881813 [Salmonella enterica subsp. enterica serovar Agona]
TTCAGCCTGATAAAGCTGTTCGCACAGCTTCTGCACCTCGTTACCGTCCGCCATTAGCACAATACACCCCTGTGAAATCT
TCACCGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAG
TAACAGGTGGTTATACAGGTTTCGCTGTTGATGTGGCGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAA
TTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAGCGAGTATGGGCGCGACATCCTGGCACCGCGCC
CCCCTCTCCCCGGCCAGCACCGGGCGGTGATGAGCAACCGGCTACCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6