Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2270244..2271163 Replicon chromosome
Accession NZ_CP069075
Organism Mycobacterium tuberculosis strain N0004

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JN138_RS10705 Protein ID WP_003900449.1
Coordinates 2270558..2271163 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN138_RS10700 Protein ID WP_003410124.1
Coordinates 2270244..2270549 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN138_RS10670 2265255..2266511 - 1257 WP_003902247.1 HNH endonuclease signature motif containing protein -
JN138_RS10675 2266865..2267440 + 576 WP_003410100.1 hypothetical protein -
JN138_RS10680 2267437..2268477 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JN138_RS10685 2268719..2269438 + 720 WP_003410108.1 DUF433 domain-containing protein -
JN138_RS10690 2269428..2269844 + 417 WP_003410114.1 hypothetical protein -
JN138_RS10695 2269860..2270159 - 300 WP_003410120.1 hypothetical protein -
JN138_RS10700 2270244..2270549 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN138_RS10705 2270558..2271163 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN138_RS10710 2271188..2271547 - 360 WP_003410131.1 hypothetical protein -
JN138_RS10715 2271707..2272297 - 591 WP_057145698.1 hypothetical protein -
JN138_RS10720 2272336..2272572 - 237 WP_003410133.1 hypothetical protein -
JN138_RS10725 2272779..2273675 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T189679 WP_003900449.1 NZ_CP069075:c2271163-2270558 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189679 NZ_CP090929:c2567207-2566923 [Salmonella enterica subsp. enterica serovar Agona]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189679 WP_003410124.1 NZ_CP069075:c2270549-2270244 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189679 NZ_CP090929:c2567445-2567197 [Salmonella enterica subsp. enterica serovar Agona]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References