Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2288745..2289664 Replicon chromosome
Accession NZ_CP069074
Organism Mycobacterium tuberculosis strain N1274

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JN139_RS10790 Protein ID WP_003900449.1
Coordinates 2289059..2289664 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN139_RS10785 Protein ID WP_003410124.1
Coordinates 2288745..2289050 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN139_RS10750 2284008..2284346 + 339 Protein_2123 hypothetical protein -
JN139_RS10760 2285705..2285941 + 237 Protein_2125 hypothetical protein -
JN139_RS10765 2285938..2286978 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JN139_RS10770 2287220..2287939 + 720 WP_003410108.1 DUF433 domain-containing protein -
JN139_RS10775 2287929..2288345 + 417 WP_003410114.1 hypothetical protein -
JN139_RS10780 2288361..2288660 - 300 WP_003410120.1 hypothetical protein -
JN139_RS10785 2288745..2289050 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN139_RS10790 2289059..2289664 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN139_RS10795 2289689..2290048 - 360 WP_003410131.1 hypothetical protein -
JN139_RS10800 2290208..2290861 - 654 WP_014324107.1 hypothetical protein -
JN139_RS10805 2290837..2291073 - 237 WP_003410133.1 hypothetical protein -
JN139_RS10810 2291280..2292176 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T189619 WP_003900449.1 NZ_CP069074:c2289664-2289059 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189619 NZ_CP090889:c1147235-1146981 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189619 WP_003410124.1 NZ_CP069074:c2289050-2288745 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189619 NZ_CP090889:c1147479-1147237 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References