Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3565811..3566481 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN140_RS16925 Protein ID WP_003899954.1
Coordinates 3565811..3566155 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN140_RS16930 Protein ID WP_003899955.1
Coordinates 3566152..3566481 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS16890 3560884..3561744 + 861 WP_003416628.1 alpha/beta hydrolase -
JN140_RS16895 3561719..3562234 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JN140_RS16900 3562250..3562461 + 212 Protein_3336 (R)-hydratase -
JN140_RS16905 3562474..3562767 + 294 WP_003416635.1 hypothetical protein -
JN140_RS16910 3563055..3564344 + 1290 WP_003416640.1 ATP-binding protein -
JN140_RS16915 3564691..3565125 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN140_RS16920 3565128..3565580 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN140_RS16925 3565811..3566155 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN140_RS16930 3566152..3566481 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN140_RS16940 3568377..3568724 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN140_RS16945 3568721..3569341 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
JN140_RS16950 3569501..3570766 - 1266 WP_003902423.1 hypothetical protein -
JN140_RS16955 3570934..3571143 + 210 WP_003416778.1 hypothetical protein -
JN140_RS16960 3571178..3571363 + 186 WP_015630737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189576 WP_003899954.1 NZ_CP069073:3565811-3566155 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189576 NZ_CP090879:326575-326763 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189576 WP_003899955.1 NZ_CP069073:3566152-3566481 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189576 NZ_CP090879:326286-326546 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References