189516

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3552575..3553245 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN141_RS16845 Protein ID WP_003899954.1
Coordinates 3552575..3552919 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN141_RS16850 Protein ID WP_003899955.1
Coordinates 3552916..3553245 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS16810 3547656..3547867 + 212 Protein_3317 (R)-hydratase -
JN141_RS16815 3547880..3548173 + 294 WP_003416635.1 hypothetical protein -
JN141_RS16820 3548461..3549597 + 1137 Protein_3319 ATP-binding protein -
JN141_RS16825 3549649..3550910 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
JN141_RS16830 3550953..3551108 + 156 Protein_3321 ATP-binding protein -
JN141_RS16835 3551455..3551889 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN141_RS16840 3551892..3552344 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN141_RS16845 3552575..3552919 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN141_RS16850 3552916..3553245 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN141_RS16855 3553783..3554130 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN141_RS16860 3554127..3554747 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
JN141_RS16865 3554907..3556172 - 1266 WP_003902423.1 hypothetical protein -
JN141_RS16870 3556340..3556549 + 210 WP_003416778.1 hypothetical protein -
JN141_RS16875 3556796..3557830 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189516 WP_003899954.1 NZ_CP069072:3552575-3552919 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189516 NZ_CP090834:c23938-23654 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189516 WP_003899955.1 NZ_CP069072:3552916-3553245 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189516 NZ_CP090834:c24170-23928 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
AlphaFold DB G0THF6

References