Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2263670..2264589 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JN141_RS10640 Protein ID WP_003900449.1
Coordinates 2263984..2264589 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN141_RS10635 Protein ID WP_003410124.1
Coordinates 2263670..2263975 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS10605 2258933..2259508 + 576 WP_003410100.1 hypothetical protein -
JN141_RS10610 2259505..2260545 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JN141_RS10615 2260670..2261931 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
JN141_RS10620 2262145..2262864 + 720 WP_003410108.1 DUF433 domain-containing protein -
JN141_RS10625 2262854..2263270 + 417 WP_003410114.1 hypothetical protein -
JN141_RS10630 2263286..2263585 - 300 WP_003410120.1 hypothetical protein -
JN141_RS10635 2263670..2263975 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN141_RS10640 2263984..2264589 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN141_RS10645 2264614..2264973 - 360 WP_003410131.1 hypothetical protein -
JN141_RS10650 2265133..2265765 - 633 WP_003913310.1 hypothetical protein -
JN141_RS10655 2265762..2265998 - 237 WP_003410133.1 hypothetical protein -
JN141_RS10660 2266205..2267101 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2257274..2272105 14831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T189498 WP_003900449.1 NZ_CP069072:c2264589-2263984 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189498 NZ_CP090792:c1338159-1337848 [Enterobacter hormaechei]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGTTC
TTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTATCGGAGAACGAAAGAATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189498 WP_003410124.1 NZ_CP069072:c2263975-2263670 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189498 NZ_CP090792:c1337851-1337432 [Enterobacter hormaechei]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CCAAAATCGATAAGTACGAAAACGAGTCCTCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References