Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3545368..3546038 Replicon chromosome
Accession NZ_CP069071
Organism Mycobacterium tuberculosis strain N0136

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN142_RS16805 Protein ID WP_003899954.1
Coordinates 3545368..3545712 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN142_RS16810 Protein ID WP_003899955.1
Coordinates 3545709..3546038 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN142_RS16770 3540441..3541301 + 861 WP_003416628.1 alpha/beta hydrolase -
JN142_RS16775 3541276..3541791 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JN142_RS16780 3541807..3542018 + 212 Protein_3315 (R)-hydratase -
JN142_RS16785 3542031..3542324 + 294 WP_003416635.1 hypothetical protein -
JN142_RS16790 3542612..3543901 + 1290 WP_003906996.1 ATP-binding protein -
JN142_RS16795 3544248..3544682 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN142_RS16800 3544685..3545137 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN142_RS16805 3545368..3545712 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN142_RS16810 3545709..3546038 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN142_RS16815 3546576..3546923 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN142_RS16820 3546920..3547540 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
JN142_RS16825 3547700..3548965 - 1266 WP_003902423.1 hypothetical protein -
JN142_RS16830 3549133..3549342 + 210 WP_003416778.1 hypothetical protein -
JN142_RS16835 3549589..3550774 - 1186 Protein_3326 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189455 WP_003899954.1 NZ_CP069071:3545368-3545712 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189455 NZ_CP090773:c1574689-1574270 [Burkholderia multivorans]
ATGATCCTTGTTGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTCGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCATGGGCCTCATAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189455 WP_003899955.1 NZ_CP069071:3545709-3546038 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189455 NZ_CP090773:c1574925-1574686 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAACTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGTGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGACGGGAGGCAGGCGGTGTCGATTTCGACATTCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References