Detailed information of TA system TA189455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3545368..3546038
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: JN142_RS16805
• Protein ID: WP_003899954.1
• Coordinates: 3545368..3545712 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: JN142_RS16810
• Protein ID: WP_003899955.1
• Coordinates: 3545709..3546038 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS16770	3540441..3541301	+	861	WP_003416628.1	alpha/beta hydrolase	-
JN142_RS16775	3541276..3541791	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
JN142_RS16780	3541807..3542018	+	212	Protein_3315	(R)-hydratase	-
JN142_RS16785	3542031..3542324	+	294	WP_003416635.1	hypothetical protein	-
JN142_RS16790	3542612..3543901	+	1290	WP_003906996.1	ATP-binding protein	-
JN142_RS16795	3544248..3544682	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
JN142_RS16800	3544685..3545137	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JN142_RS16805	3545368..3545712	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN142_RS16810	3545709..3546038	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
JN142_RS16815	3546576..3546923	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
JN142_RS16820	3546920..3547540	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
JN142_RS16825	3547700..3548965	-	1266	WP_003902423.1	hypothetical protein	-
JN142_RS16830	3549133..3549342	+	210	WP_003416778.1	hypothetical protein	-
JN142_RS16835	3549589..3550774	-	1186	Protein_3326	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T189455 WP_003899954.1 NZ_CP069071:3545368-3545712 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T189455 NZ_CP090773:c1574689-1574270 [Burkholderia multivorans]
ATGATCCTTGTTGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTCGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCATGGGCCTCATAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT189455 WP_003899955.1 NZ_CP069071:3545709-3546038 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT189455 NZ_CP090773:c1574925-1574686 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAACTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGTGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGACGGGAGGCAGGCGGTGTCGATTTCGACATTCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6