Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2258636..2259555 Replicon chromosome
Accession NZ_CP069071
Organism Mycobacterium tuberculosis strain N0136

Toxin (Protein)


Gene name HigB2 Uniprot ID A0A654U351
Locus tag JN142_RS10615 Protein ID WP_003906719.1
Coordinates 2258950..2259555 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN142_RS10610 Protein ID WP_003410124.1
Coordinates 2258636..2258941 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN142_RS10580 2253647..2254903 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
JN142_RS10585 2255257..2255832 + 576 WP_003410100.1 hypothetical protein -
JN142_RS10590 2255829..2256869 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JN142_RS10595 2257111..2257830 + 720 WP_003410108.1 DUF433 domain-containing protein -
JN142_RS10600 2257820..2258236 + 417 WP_003410114.1 hypothetical protein -
JN142_RS10605 2258252..2258551 - 300 WP_003410120.1 hypothetical protein -
JN142_RS10610 2258636..2258941 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN142_RS10615 2258950..2259555 - 606 WP_003906719.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN142_RS10620 2259580..2259939 - 360 WP_003410131.1 hypothetical protein -
JN142_RS10625 2260099..2260689 - 591 WP_057145698.1 hypothetical protein -
JN142_RS10630 2260728..2260964 - 237 WP_003410133.1 hypothetical protein -
JN142_RS10635 2261171..2262067 - 897 WP_003906720.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22716.91 Da        Isoelectric Point: 7.3073

>T189437 WP_003906719.1 NZ_CP069071:c2259555-2258950 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189437 NZ_CP090766:1959992-1960162 [Burkholderia multivorans]
GTGCTCGACGAAGGGGTCTGCGAACTCAAGATCGACTTTGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGT
GGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCAACGCAACGGAAGGACGTCAAGCGCGCCGTGCAGATGGCGCGCGAAC
TGAAGGAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189437 WP_003410124.1 NZ_CP069071:c2258941-2258636 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189437 NZ_CP090766:1960166-1960450 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGCCGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCAAGGTCGCGCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A654U351

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References