Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3564593..3565263 Replicon chromosome
Accession NZ_CP069070
Organism Mycobacterium tuberculosis strain N1283

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN143_RS16840 Protein ID WP_003899954.1
Coordinates 3564593..3564937 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN143_RS16845 Protein ID WP_003899955.1
Coordinates 3564934..3565263 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN143_RS16805 3559666..3560526 + 861 WP_003416628.1 alpha/beta hydrolase -
JN143_RS16810 3560501..3561016 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JN143_RS16815 3561032..3561243 + 212 Protein_3321 (R)-hydratase -
JN143_RS16820 3561256..3561549 + 294 WP_003416635.1 hypothetical protein -
JN143_RS16825 3561837..3563126 + 1290 WP_003416640.1 ATP-binding protein -
JN143_RS16830 3563473..3563907 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN143_RS16835 3563910..3564362 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN143_RS16840 3564593..3564937 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN143_RS16845 3564934..3565263 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN143_RS16850 3565801..3566148 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN143_RS16855 3566145..3566765 + 621 WP_031714159.1 RES family NAD+ phosphorylase -
JN143_RS16860 3566925..3568190 - 1266 WP_003902423.1 hypothetical protein -
JN143_RS16865 3568358..3568567 + 210 WP_003416778.1 hypothetical protein -
JN143_RS16870 3568602..3568787 + 186 WP_003908177.1 hypothetical protein -
JN143_RS16875 3568814..3569848 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189394 WP_003899954.1 NZ_CP069070:3564593-3564937 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189394 NZ_CP090754:c628886-628308 [Burkholderia multivorans]
ATGAACCCTGACACGATCCGCGTACGGCTCGATTTCCTGCGCGAAGCCGAACGCCTGAAGGACGTGCTGCGCAGCGGCCG
CACGTCGTCCGGTCGCCGCGAAAGCACCGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTAGTGTTCGCCGACGCAC
TGCCCGGCATCGACACGCTGAAGCTGCTCAAGCTGTGCGTCGTCCACGATCTCGGCGAGGCGCTGCACGGCGACATACCG
GCGACCGAGCAAGCCGCGCATCCGGACAAGAACATGCACGAACGCGACGACCTGCTCACGTTGACGGCCGCACTCGATCC
CGTGCTGCGTAACGAGCTCGTCGCACTGTGGGACGAATACGACGCGGCCGCGTCGCCCGAAGCGCGCGCCGCGAAGGCGC
TCGACAAGCTCGAGACGATTCTGCAGCACACGCAGGGCGCCAACCCGCCCGACTTCGACTACGCGTTCAATCTCGACTAC
GGCCGGCGCTATACGGATGCGGCACCGCTGTTCAGCGCGATCCGCGCGATCGTCGATGCGGACACGCAGCGCAGGATCGA
CGCGCAAGCACAAACGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189394 WP_003899955.1 NZ_CP069070:3564934-3565263 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189394 NZ_CP090754:c629578-629021 [Burkholderia multivorans]
ATGCCCGATTCGCCGCTTCATACGCCGCCGCTGGGCCCGCAGCTGAAACGCTGGCGCCTGCTGCATCGCGTCAAGCAAAG
CCATGCCGCCGAGCTGTTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCGTCCAGCAGATGTCGCCCGACG
AGCGCGCGACGGCCGAACGCCTGCTCGCCGCGCACCTCGACTCCGCGGGCGACCATGCGCTCGCGCGGCTGGTCGCAGGG
AGCGCGGGCCGCATGCATCTCGTCTGCGATCTGACGCACCGGCTGCTCGCATGCTCGCCGTCTCGCGCAGCCGAGTTCTC
GCAGCCGCTGTCGATGCTGCTCGGCACGTCGCTGTGGCGCTATGCGACAGAAGAGATCGTCCGGATGGAAACCGCACTGG
ACACACTCGGCTGGCACGATTGCGCAGGACCGCCGAGCGTCGAATTCGACACCGGCACGAACGCGTCGCGCGTCGTGCCG
ATCCACCGCAGCCGATGCCGCTGGACGCGGATCATGCTGTCGGACGGATCGGCGGCACGGCTCGTCGAAACGCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References