Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2410623..2411542 Replicon chromosome
Accession NZ_CP069070
Organism Mycobacterium tuberculosis strain N1283

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JN143_RS11295 Protein ID WP_003900449.1
Coordinates 2410623..2411228 (+) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN143_RS11300 Protein ID WP_003410124.1
Coordinates 2411237..2411542 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN143_RS11275 2408111..2409007 + 897 WP_003410136.1 hypothetical protein -
JN143_RS11280 2409214..2409450 + 237 WP_003410133.1 hypothetical protein -
JN143_RS11285 2409489..2410079 + 591 WP_057145698.1 hypothetical protein -
JN143_RS11290 2410239..2410598 + 360 WP_003410131.1 hypothetical protein -
JN143_RS11295 2410623..2411228 + 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN143_RS11300 2411237..2411542 + 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN143_RS11305 2411627..2411926 + 300 WP_003410120.1 hypothetical protein -
JN143_RS11310 2411942..2412358 - 417 WP_003410114.1 hypothetical protein -
JN143_RS11315 2412348..2413067 - 720 WP_003410108.1 DUF433 domain-containing protein -
JN143_RS11320 2413309..2414349 - 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JN143_RS11325 2414346..2414921 - 576 WP_003410100.1 hypothetical protein -
JN143_RS11330 2415275..2416530 + 1256 Protein_2235 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T189375 WP_003900449.1 NZ_CP069070:2410623-2411228 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189375 NZ_CP090746:2031070-2031354 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGACGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189375 WP_003410124.1 NZ_CP069070:2411237-2411542 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189375 NZ_CP090746:2030773-2031066 [Burkholderia multivorans]
ATGGTTACTTTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCGC
AGCCCGCCTGATCAGCGCGCAACTCGGCAACTTCGGCGAGTACCGTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCG
ATTTCGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCA
ACGCAACGGAAGGACATCAAGCCCGTGCAGATGGCGCGCGAACTGAAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References