Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2217445..2218313 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag JN144_RS10370 Protein ID WP_010886136.1
Coordinates 2217445..2217822 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag JN144_RS10375 Protein ID WP_003409886.1
Coordinates 2217864..2218313 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS10320 2213234..2213686 - 453 WP_003899095.1 lipoprotein -
JN144_RS10325 2213750..2214151 + 402 WP_003409869.1 hypothetical protein -
JN144_RS10330 2214144..2214326 - 183 WP_003409870.1 hypothetical protein -
JN144_RS10335 2214440..2214790 - 351 WP_003409871.1 hypothetical protein -
JN144_RS10340 2214801..2215703 - 903 WP_003409874.1 hypothetical protein -
JN144_RS10345 2215724..2215915 - 192 WP_003409876.1 hypothetical protein -
JN144_RS10350 2215916..2216212 - 297 WP_003409877.1 hypothetical protein -
JN144_RS10355 2216452..2216667 + 216 WP_003409878.1 antitoxin -
JN144_RS10360 2216664..2216975 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
JN144_RS10365 2216949..2217470 - 522 WP_003904745.1 hypothetical protein -
JN144_RS10370 2217445..2217822 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
JN144_RS10375 2217864..2218313 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JN144_RS10380 2218310..2218855 + 546 WP_003409891.1 SecB-like chaperone -
JN144_RS10385 2218744..2219358 - 615 WP_003901296.1 hypothetical protein -
JN144_RS10390 2219407..2219703 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
JN144_RS10395 2219700..2219951 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
JN144_RS10400 2219938..2220432 + 495 WP_003899099.1 hypothetical protein -
JN144_RS10405 2220592..2220999 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
JN144_RS10410 2221003..2221275 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN144_RS10415 2221308..2222528 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T189309 WP_010886136.1 NZ_CP069069:2217445-2217822 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T189309 NZ_CP090727:1272694-1272990 [Burkholderia multivorans]
ATGGAAAAGCGAACGGCGCACTGCAAGCTGCCGCGCGTCAAGGCGTTGATCGAAGCCGGCAAGGTCCGCCTGACCGCAAG
CGCCGTCATCGGCGCCCGCCTGATGGGCTTCACCGAAGCGGAAGCGATCGACGTGGTGAGGGCGCTGACACCGTTCGAAT
TCCACAAGAGCATGACGACCTACGCCGACCACACGATCTGGCAGGACGTCTACCGGCCTTGCACGATGCGCGGCGACGTG
TACCTGAAACTGACCGTCGTCGACGATGTGCTGATCGTGTCTTTCAAGGAGCGATGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT189309 WP_003409886.1 NZ_CP069069:2217864-2218313 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT189309 NZ_CP090727:1272992-1273393 [Burkholderia multivorans]
ATGAAATGTCCTGCATGCGGTGCTGCCACGCTGGTCCGCGACACGCGCGACATCCCGTACACCTACAAGGGCGAATCGAC
CGTGATCCCGCACATCACCGGCGAATTTTGCCCCGCGTGCGGCGAGTCGGTGCTCGACGTCGACGAGGCGACCCGTTTCG
GCGAAGCGGTGTCGGCGTTCAACCGGCAGGTGAACGCGACACTCGTCGATCCGAAGTTCATTGCGACGGTGCGCAAGAAG
CTGAAGCTCGATCAGCGCGAGGCGGCCGAGATTTTCGGCGGCGGCGTCAACGCGTTTTCCCGCTACGAGACCGGCAAGAC
CAATCCGCCGCTGGCGCTCGTGAAGCTGCTGAAGATTCTCGACCGTCATCCCGATCTGCTCGACGAAGTGCGTGCGGCAT
AA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References